簡便な血小板製剤中の微量混入白血球測定法

概要

論文の詳細を見る
As the technology for leukoreduction from blood products is advanced, sensitive and reliable methods to count extremely low number of leukocytes are expected to be developed. we evaluated IMAGN 2000(IMAGN)for the measurement of residual leukocytes in leukoreduced blood products. IMAGN is an automated volumetric capillary cytometer utilizing dyelabeled antibodies and He-Ne laser. The performance of IMAGN was tested with samples adjusted to 0.1-100/μL leukocytes from platelet concentrates(PC)as well as PC samples derived from apheresis and compared with those of Nageotte hemocytometry and flow cytometry. The reproducibility, linearity and sensitivity of the IMAGN was comparable with the two other methods at 0.1-100/μL of leukocyte concentration. The correlation coefficient(r^2)between expected and observed number of leukocytes was 0.997, 0.993 and 1.000, respectively for IMAGN, Nageotte and flowcytemeter. The coefficient of variation with IMAGN was 38.6%, 26.6% and 12.5% at the concentration of 0.1, 1 and 10/μL of leukocytes, respectively. Clear linear relationships of the leukocyte counts were observed between IMAGN and the two other methods with apheresis sample. In addition, IMAGN is advantageous over the two other methods with respect to sample preparation, measuring time and easy operation. Since 3-10/μL of residual leukocytes or less are required as final concentration after leukoreduction and IMAGN showed good performance at 0.1-10/μL of leukocyte concentration, IMAGN is sensitive and reliable enough for the measurement of residual leukocytes in leukoreduced PC in routine work.
日本医療機器学会の論文
2001-09-01

簡便な血小板製剤中の微量混入白血球測定法

スポンサーリンク

概要

著者

関連論文

スポンサーリンク