クリオ-フィブリングルーの調製法 : 全自動クリオプレシピテート調製装置(CryoSeal^<TM> System)に関する検討(セミナー「ストレスと生物の応答・適応 : その分子・細胞機構と応用」)

概要

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Fibrin glue is a topical biological adhesive widely used in surgery for tissue adhesion and wound healing. The effect of fibrin glue imitates the final stages of coagulation. We prepared cryo-fibrin glue from autologous blood using the CryoSeal System. We chose the CryoSeal^<TM> System because the cryo-fibrin glue can be produced in a automated, rapid way. In fact, the entire process only required about 56 minutes. After separating fresh plasma (average volume: 199.2±1.8mL) from whole blood, cryoprecipitate was extracted. The concentration and volume of fibrinogen were 33.4±7.5mg/mL and 3.9±0.9mL, respectively (n=9), and the yields of fibronectin, plasminogen, and blood coagulation factor XIII were 10.9±1.4mg/mL, 177±51%, and 761±310%, respectively (n=9). The tensile strength of fibrin glue when mixed with thrombin was comparable to that of commercialy available fibrin glue. This system may be useful to prepare cryo-fibrin glue from autologous blood at the medical facilities.
低温生物工学会の論文
2003-08-30