大容量凍結保存装置を用いた造血幹細胞の凍結保存

概要

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A large number of CB units have been cryopreserved to promote CB transplantation as a source of allogeneic hematopoietic stem cell transplantation, and the management of cryopreservation for these units has come to an important issue. We evaluated the efficacy of a newly-developed cryopreservation system, N2BioArchive^<TM>, that is capable of storing a large number of CB units and handling units with barcode system. N2BioArchive system has a capacity to store up to 3,626 units in the inner space (diameter 130cm, height 125cm) and has a rate-controlled freezing system. The volume of white blood cells fraction derived from peripheral blood (PB) and CB was adjusted to 25ml with 10% dimethylslufoxide and 1% dextran. The time for accomplishing the freezing procedure for one unit was about 26 minutes. After thawing, the cell viability of PB and CB was 73.9±6.7%, 92.0±9.1% respectively. The recovery of colony forming cells of PB and CB was 66.4±12.7%, 94.1±13.7% respectively. The cell viability at recovery of colony forming cells in N2BioArchive system was comparable to the conventional method. Thus, N2BioArchive is available for the cryopreservation of umbilical cord blood cells.
日本医療機器学会の論文
2001-01-01