Immunohistochemical Localization of Prostaglandin F_<2α> in Reperfused Gerbil Brain

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Immunohistochemical localization of prostaglandin (PG) F_<2α> synthesized during recirculation following experimental forebrain ischemia was studied in Mongolian gerbils. The bilateral carotid arteries were clamped for 5 minutes, and the brains were frozen in situ after 5 minutes of recirculation. Shamoperated gerbils not subjected to arterial occlusion served as controls. Cryostat sections containing dorsal hippocampus 10 μm in thickness were incubated in rabbit anti-PGF_<2α> serum and stained with avidin-biotin-peroxidase complex after fixation in carbodi-imide and Zamboni's solution. In specimens from control animals, blood vessels were faintly stained with anti-PGF_<2α> serum, while neurons and glial cells were less intensely stained or unstained. Specimens from reperfused gerbils demonstrated strongly positive staining for PGF_<2α> in blood vessels, neurons (especially hippocampal pyramidal neurons), and glial cells. Staining for PGF_<2α> in the reperfused animals was substantially diminished by pretreatment with indomethacin (10 mg/kg, i.p.). The results indicate that recirculation after forebrain ischemia results in an increase in PGF_<2α> in neurons, glial cells, and blood vessels.
日本脳神経外科学会の論文
1990-04-15