TRANSFERRIN RECEPTOR MOLECULES IN HUMAN CELL LINES DERIVED FROM THE CNS MALIGNANT GLIOMAS : IMMUNOHISTOCHEMICAL AND FLOW-CYTOMETRIC STUDY (<Symposium II>Modern Histo- and Cytochemistry in Neuroscience : Embryogenesis and Regeneration of the Nervous System
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概要
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The ten human cell lines derived from glioma tissues were characterized for the aspects of the phenotypic expression of GFAP and S-100 protein, DNA-ploidy, doubling time, and response to DBc-AMP and LAK cells. The expression of the TfR was evaluated on these established cell lines with correlation to their phenotypic markers and characteristics. As a result of immunohistochemical studies on frozen materials using a monoclonal anti-TfR antibody, the high expression of the TfR was related to the doubling time (36-48 hr), but not to the phenotypic expression of glial markers (GFAP and S-100 protein) and other characteristics. And thus, rapidly proliferating astrocytic cell lines including C6 and U251 showed the high level of the TfR in the cells. For the flow cytometry (FCM), the cell harvest with trypsin abolished the extracellular part of TfR molecules from the cell membrane, and thus, single cell preparation with trypsin was not appropriate for the study. On the other hand, EDTA (0.02%) with mechanical shaking provided a preservation of the TfR molecules for FCM study. The series of FCM analysis demonstrated that newly synthesized (or recycled) TfR molecules appear in the extracellular matrix of the cell membrane by 12 hr and then increase in quantity up to 28 hr after trypsin digestion. The results suggest that the TfR molecules are functioning in rapidly proliferating cells derived from the central nervous system as described previously in other types of tissue.
- 日本組織細胞化学会の論文
著者
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Takakura Kintomo
Department Of Neurosurgery Faculty Of Medicine University Of Tokyo
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Takakura Kintomo
Department Of Neurosurgery University Of Tokyo Hospital:m.d. Department Of Neurosurgery Neurological
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Nakamura Hirohiko
Department Of Neurosurgery Nakamura Memorial Hospital
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Nakamura Hirohiko
Department Of Neurosurgery University Of Tokyo Hospital:m.d. Department Of Neurosurgery Tokyo Metrop
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Shitara Nobuyuki
Department Of Neurosurgery University Of Tokyo Hospital:m.d. Department Of Neurosurgery Tokyo Metrop
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Shitara Nobuyuki
Department Of Neurosurgery Tokyo Metropolitan Komagome Hospital
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KITAMURA KAZUYUKI
Department of Neurosurgery, University of Tokyo
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WADA TERUMI
Department of Neurosurgery, University of Tokyo
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Takakura Kintomo
Department Of Neurosuegery National Cancer Center Hospotal
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Wada Terumi
Department Of Neurosurgery University Of Tokyo
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Kitamura Kazuyuki
Department Of Neurosurgery University Of Tokyo
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