Characteristics of an Established Human Glioma Cell Line, KNS-42
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概要
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We established a human glioma cell line derived from a malignant glioma and evaluated it by immunochemical techniques and antibodies to astroglial (glial fibrillary acidic protein: GFAP, S-100 protein), oligodendroglial (myelin basic protein, galactocerebroside), neuronal (neuron-specific enolase: NSE, neurofilament triplet proteins), and mesenchymal (vimentin, fibronectin) antigens. The cell line was epithelial in sparse culture and glial in dense culture. GFAP was expressed by most cells in sparse culture and primarily by overlying cells in dense culture. The amount of GFAP depended on the cell density and ranged from 530 to 990 ng/mg of protein. The cells contained much more vimentin than GFAP. Neurofilament proteins, S-100 protein, myelin basic protein, and galactocerebroside were undetectable. The cells synthesized a small amount of fibronectin and released it into the medium. The amount of NSE was unrelated to cell density, ranged from 13 to 19 ng/mg of protein, and was present in greater quantity in cells cultured in a medium containing 1 μl/ml of sodium lactate. We conclude that KNS-42 is a permanent astrocytic glioma cell line; it expresses GFAP, NSE, vimentin, and fibronectin, and is morphologically and functionally dependent on its environment.
- 日本脳神経外科学会の論文
- 1987-07-15
著者
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NAGASAKA Susumu
Department of Neurosurgery, Fukuoka University School of Medicine
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KITAMURA Katsutoshi
Department of Neurosurgery, Neurological Institute, Kyushu University
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TAKAKI Tosuke
Department of Neurosurgery, Neurological Institute, Kyushu University
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Nagasaka Susumu
Department Of Neurosurgery Fukuoka University School Of Medicine
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Nagasaka Susumu
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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TAKESHITA Iwao
Departments of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University
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KURAMITSU Masaharu
Departments of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University
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MACHI Takao
Departments of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University
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OGAWA Hisayuki
Departments of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University
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EGAMI Hiroto
Departments of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University
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MANNOJI Hiromichi
Departments of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University
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FUKUI Masashi
Departments of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University
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Machi Takao
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Egami Hiroto
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Fukui Masashi
Department Of Neurosurgery Kyushu University Faculty Of Medicine
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Fukui Masashi
Department Of Neurosurgery Graduate School Of Medical Science Kyushu University
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Takaki Tosuke
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Takaki Tosuke
Department Of Neurosurgery Faculty Of Medicine Kyushu University
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Takeshita I
九州労災病院
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Takeshita Iwao
Department Of Neurosurgery Faculty Of Medicine Kyushu University
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Ogawa Hisayuki
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Ogawa Hisayuki
Department Of Neurological Surgery Saga Prefectural Hospital Koseikan
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Mannoji Hiromichi
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Mannoji Hiromichi
Department Of Neurosurgery Yamaguchi Red Cross Hospital
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Kuramitsu Masaharu
Departments Of Neuropathology Neurological Institute Faculty Of Medicine Kyushu University
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Kitamura Katsutoshi
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Kitamura Katsutoshi
Department Neurosurgery Kyushu University
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Fukui Masashi
Department Of Neurosurgery Neurological Institute Graduate School Of Medical Sciences Kyushu Univers
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Fukui Masashi
Departments Of Neurosurgery Neurological Institute Faculty Of Medicine Kyushu University
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Fukui Masashi
Department Of Neurosurgery Faculty Of Medicine Kyushu University
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