60 内生放線菌Streptomyces galbus R-5株処理による耐病性シャクナゲ組織培養苗の育成

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Streptomyces galbus R-5, of the intense antagonistic activity against Pestalotiopsis sydowiana and Phytophthora cinnamomi, major pathogens of rhododendron, was used as a biocontrol agent. An appropriate amount of the mycelial suspension of R-5 was spread on the surface of multiplication medium in glass flasks where tissue-cultured rhododendron seedlings were growing The seedlings treated and untreated with R-5 were incubated a further 10 days in an illuminated incubator. The following inoculation tests were performed to examine whether resistance was induced in R-5 treated seedlings. (i) A mycelial disk of P.sydowiana was placed on the upper 4th leaves of treated and untreated seedlings, followed by incubation for 14 days. (ii) R-5 treated and untrealed seedlings were transplanted to soil in plastic boxes that was amended with an appropriate number of mycelial disks of Phy. cinnamomi. In 92% of R-5 pretreated seedlings only inoculated leaves showed a brownish symptom, but mycelia never spread to other leaves from the inoculated leaves. In 36% of untreated seedlings, leaves lower and upper than the inoculated leaves brownish and in addition 54% of seedlings entirely died. In the transplant experiment, 34% ofuntreated seedlings were killed by Phy. cinnamomi, while only 15% of treated seedlings became dead within a few weeks after transplant. These results show that pretreatment of tissue-cultured rhododendron seedlings with R-5 induced an intense resistance to both pathogens and that such an resistance is effective in tissue-culture flasks and even after transplanting in soil.
植物化学調節学会の論文
2001-10-09

60 内生放線菌Streptomyces galbus R-5株処理による耐病性シャクナゲ組織培養苗の育成

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