Exo-β-glucosaminidase産生菌の分離

概要

論文の詳細を見る
Sixty-eight strains, grown on agar plate containing chitosan as a carbon source, were isolated from twenty soil samples. Based on the investigation about the characteristic features of sugar products from chitosan, the isolated strains were classified into the following five groups. Sugar product from chitosan was consist of (1) only glucosamine, (2) only chitobiose, (3) glucosamine and chitobiose, (4) chitoologosaccharides, (5) glucosamine and chitooligosaccharides. From the three strains belong to the group (1), No.CC6-7 strain showing the highest productive of chitosan-hydrolyzing enzyme, was selected. Sugar chitosan with the culture filtrate of No.CC6-7 strain was only glucosamine throughout the entire course of hydrolysis, and the strain did not form halo in chitosan-agar plate.these facts strongly suggest that the chitosan hydrolyzing enzyme produced by this strain is only exo-β-glucosaminidase.
実践女子大学の論文
2005-03-25