いんげんまめ(Phaseolus vulgaris L)の糖質分解酵素:ガラクタナーゼの精製および性質

概要

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Galactanase activity in a homogenized preparation of the kidney beans, Phaseolusvulgaris L was examined together with the other carbohydrase activities. This enzymewas purified by the affinity column and gel-filtration column chromatography. SDSPAGEshowed the enzyme had molecular weight of 34kDa. The enzyme hydrolyzed the substrate galactan by endo-type of action and produced several galacto-oligosaccharides.Because the substrate galactan used in this experiment was isolated from the gum arabic,which contained β-1, 3 - galactan as a major component, this enzyme wouldcorrespond to β- 1, 3 - galactanase.
2012-03-10