キトサン加水分解酵素産生微生物のスクリーニング

概要

論文の詳細を見る
Sixty-three strains, grown on agar plate containing chitosan as a carbon source, were isolated from twenty-one soil samples. Based on the investigation about the productivity of chitosan-hydrolyxzing enzymes and the characteristic features of sugar products from chitosan, two strains, C6-2 and C11-1, were selected from the isolated sixty-three strains. The C11-1 strain formed clear halo in chitosan-agar plate and hydrolysis products of chitosan with C11-1 enzyme were glucosamine, chitobise, and higher chitooligosaccharides, indicating an endo-chitosanase producing microorganism. The C6-2 strain did not form halo in chitosan-agar plate and hydrolysis product of chitosan with C6-2 enzyme was only chitobiose throughout the entire couse of hydrolysis. These facts indicate that the C6-2 enzyme is exo-chitobiohydrolase has not been confirmed to date, the chitosan-hydrolyzing enzyme of C6-2 strain seems to be a novel enzyme.
実践女子大学の論文
2003-04-05