Transgenic Plant Regeneration Through Silicon Carbide Whisker-Mediated Transformation of Rice (Oryza sativa L.)
スポンサーリンク
概要
- 論文の詳細を見る
We developed a rice (Oryza sativa) transformation system using silicon carbide whiskers (SCW). The scutellar tissues of rice embryos obtained from mature seeds were vortexed in liquid medium containing plasmid DNA and SCW. The embryos treated with a plasmid DNA, pAct1-F containing the β-glucuronidase (gusA) gene, transiently expressed GUS activity at a mean number of 302 spots per 250 mg sample (around 20 embryos) in optimal conditions for DNA delivery. On the other hand, the bialaphos-resistant calli were obtained from embryos treated with two plasmid DNAs, pAct1-F and pDM302 containing the bar gene, of which product confers resistance to bialaphos. Some of the resistant calli also expressed GUS activity. Fertile transgenic rice plants were regenerated from them. The integration and inheritance of bar gene were confirmed by Southern analysis of R_0 and R_1 plants.
- 日本育種学会の論文
著者
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田中 修
甲南大・理工・生物
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TANAKA Osamu
Department of Biology, Faculty of Science and Engineering, Konan University
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Tanaka O
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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鎌田 喜一郎
長岡技術科学大学化学系
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OTANI Motoyasu
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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Wakita Y
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Shimada T
Research Institute Of Agricultural Resource Ishikawa Agricultural College
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Otani M
Ishikawa Agriculture Coll. Ishikawa Jpn
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Otani Motoyasu
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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Shimada T
Esearch Institute Of Agricultural Resources Ishikawa Agricultural College
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WAKITA Yohichi
Research Institute of Agricultural Resources, Ishikawa Agricultural College
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Wakita Y
Cell Manipulation Laboratory Bio-resources Technology Division Forestry And Forest Products Research
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Wakita Yohichi
Research Institute Of Agricultural Resources Ishikawa Agricultural College
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MATSUSHITA Junjo
Department of Biology, Faculty of Science and Engineering, Konan University
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Tanaka O
Department Of Biology Faculty Of Science And Engineering Konan University
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Tanaka O
Shimane Medical University
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Shimada Takiko
esearch Institute of Agricultural Resources, Ishikawa Agricultural College
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Tanaka Osamu
Department Of Anatomy Shimane Medical University
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Tanaka O
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Matsushita Junjo
Department Of Biology Faculty Of Science Konan University
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Otani Motoyasu
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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