高速液体クロマトグラフィーによる血清中塩酸ピルジカイニドの定量とTDMへの応用

概要

論文の詳細を見る
A micro method for the determination of pilsicainide hydrochloride, an antiarrhythmic agent, in human serum was developed. In this method, serum containing the drug was extracted with benzene under alkaline conditions and then the extract was analyzed by high-performance liquid chromatography using a 5 mm Shim-pack HRC-CN column (150 mmX4.6 mm I.D.) at 40℃The mobile phase was 65 mM sodium perchlorate-acetonitrile (85 : 15). The flow-rate was kept at 1 .2 ml/min and the column effluent was monitored at 200 nm. Lidocaine hydrochloride or disopiramide phosphate was used as an internal standard. The method is accurate and reproducible for routine monitoring of serum pilsicainide hydrochloride. The serum levels of pilsicainide hydrochloride in 30 patients monitored at 1.2 to 15.5 hrs after the last dose prior to blood sampling varied from 0.18 to 1.52μg/ml. Relatively large interindividual differences were observed in the apparent total body clearances and elimination half-Iives estimated using a computer program (PEDAL). We recommend that the serum levels of pilsicainide hydrochloride be monitored in patients receiving this drug therapy.
日本医療薬学会の論文
1997-10-10