モノクローナル抗体を用いたFPIA法による全血中シクロスポリン濃度モニタリング

概要

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The clinical advantage of fluorescence polarization immunoassay for determination of cyclosporine (CYA) based on monoclonal antibody (M-FPIA) was compared with high-performance liquid chromatography (HPLC), radio immunoassay on monoclonal antibody (M-RIA), and fluorescence polarization immunoassay on polyclonal antibodies (P-FPIA). To clarify the evaluation of this method (M-FPIA), whole blood levels of CYA in 24 renal transplant recipients, who were treated by CYA therapy, were determined by each analytical method. The coefficients of variation obtained by M-FPIA for the within-run and between-run precisions were 2.1 to 2.5% and 1.5 to 3.2%, respectively. There was a significantly good correlation between the data of HPLC and those of M-FPIA (r=0.988), M-RIA and M-FPIA (0.988), P-FPIA and M-FPIA (0.942), however, the value of CYA by P-FPIA was 2-fold of the value of the drug by M-FPIA. The values with M-FPIA were approximately the same as those with HPLC and M-RIA. These results suggest that M-FPIA be very useful for the emergent and routine monitoring of CYA on the clinical practice.
日本医療薬学会の論文