<研究論文>組織工学による骨誘導に関する研究

概要

論文の詳細を見る
In this study we evaluated the behavior of tissue engineering technique used rat bone marrow cells on hydroxyapatite (HAP) and calcium phosphate cement (CPC) surfaces during regeneration in bone defects. HAP or CPC, and cells extracted from bone marrow were cultured with dexamethazone for 2 weeks. The implants of CPC or HAP-cell complex were embedded in bone defects drilled at the rat calvaria. Control experiments were performed implants of HAP or CPC without cell culture. At 2 weeks of the implantation with CPC-cell complex, a thin layer of connective tissue was formed around the implants. At 4 weeks the CPC-cell complex was covered by thick layer of connective tissue. In the control experiments, the connective tissue layer was not formed. In the implant of HAP-cell complex, the amount of formation of connective tissue layer was much less than those of CPC-cell complex. The immunohistchemical studies of BMP-2,BMPR-IA and BMPR-IB showed the same distributions. In the implants of CPC or HAP-cell complex, intense immunoreactivities were detected in the osteoclasts, fibroblasts around the implants. In the control experiments, the immunoactivities of cells were weaker than those of CPC or HAP-cell complex. These results suggested that the tissue engineering method used CPC or HAP-bone marrow derived cell complex induced the connective tissue formation. BMP-2,BMPR-IA and BMPR-IB act as mainly autocrine during the connective tissue formation after the implants.
2002-12-25

<研究論文>組織工学による骨誘導に関する研究

スポンサーリンク

概要

著者

関連論文

スポンサーリンク