クロムイオンが口腔由来上皮細胞株(Ca 9-22)の生細胞数およびサイトカイン産生に及ぼす影響

概要

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The present study investigated the effects of chromium ion on cell viability, interleukin (IL)-1 α and IL-8 production by an oral epithelial cell line, Ca 9-22. The cells were maintained in DMEM supplemented with 10% FBS and antibiotics. The cultures having 100% coufluent cells were serum starved for 24 h, and then 1 mM of CrCl_3 were added for the 10 min, 30 min. 1h, 3h or 6h, respectively. After the incubation for 24 h, the cell viability was assessed by colorimetric assay using a LDH Cytotoxic Test. And also IL-1 α and IL-8 production by these cells were examined using specific ELISA kits for IL-1 α and IL-8. By the addition of 1 mM chromium ion did not alter cell viability within 1 h. However., 1mM chromium ion induced cell death more than 6 h. Little effect was observed by the addition ofchromium ion on IL-1 α and IL-8 production of these cells. These results indicate that additions of 1 mM chromium ion induce epithelial cell death rather than cytokine production.
日本大学の論文
2003-12-25