<研究論文>亜鉛イオンが口腔由来上皮細胞 (Ca 9-22) の生細胞数, DNA 合成能およびサイトカイン産生に及ぼす影響

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The present study investigated the effects of zinc ion on cell viability, DNA synthesis, interleukin (IL)-1α and IL-8 production by an oral epithelial cell line, Ca 9-22. The cells were maintained in DMEM supplemented with 10% FBS and antibiotics. The cultures having 60% or 100% coufluent cells were serum starved for 24h, and then 0,0.001,0.1 or 1 mM of ZnCl_2 were added. After the incubation for 1,6 or 24h, the cell viability was assessed by colorimetric assay using a Cell Counting Kit, and DNA synthesis was monitored by the incorporation of [^3H] -thymidine into these cells. IL-1α and IL-8 production by these cells were also examined using specific ELISA kits. By the addition of 0.01 or 0.1 mM zinc ion did not alter cell viability, however, 1mM zinc ion induced cell death. DNA synthesis of these cells were decreased by the addition of zinc ion dose dependenthy when the cells were 60% confluence, whereas 100% confluent cells were resistant until 0.1mM zinc ion. Little effect was observed by the addition of zinc ion on IL-1α and IL-8 production by these cells. These results indicate that high concentrations of zinc ion induce epithelial cell death rather than cytokine production.
日本大学の論文
2002-12-25