<研究論文>口腔由来上皮細胞株 (Ca 9-22) の生細胞数および DNA 合成能に及ぼす金属イオンの影響

概要

論文の詳細を見る
The present study investigated the effects of several dental alloy-associated metal ions on cell viability and DNA synthesis of an oral epithelial cell line, Ca 9-22. The cells were maintained in DMEM supplemented with 10% FBS and antibiotics. Confluent cultures were serum starved for 24h, and then 0 to 2mM of TiCl_4,NiCl_2 and ZnCl_2 were added. After the incubation for 24h, the cell viability was assessed by colorimetric assay using a Cell Counting Kit, and DNA synthesis was monitored by the incorporation of [^3H]-thymidine into these cells. By the addition of 0.01 to 2mM titanium ion did not alter the cell viability, but 2mM titanium ion induced significant DNA synthesis in these cells. The cell viability decreased to 50% by the addition of 2 mM nickel ion, however, DNA synthesis decreased by the addition of 0.05mM or above titanium ion dose dependently. The addition of 1mM or above concentrations of zinc ion induced significant cell death, and 0.5mM or above zinc ion induced significant decrease in DNA synthesis. These results indicate that high concentrations of titanium ion induce DNA synthesis by these cells, whereas high concentrations of nickel and zinc ions induce epithelial cell death.
日本大学の論文
2001-12-25