<研究論文>歯胚の成長に伴う硬組織形成の微細構造

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The present study investigated the effect of IL-1β in the development of tooth germ by organ culture on the basis of electron microscopic observation. The first molars (M1) from 18-day old mouse embryos were cultured in vitro by floatation method. In this method consisted of using a 24-well culture dish and a culture chamber with a membrane filter was used. The incubation atmosphere consisted 50% O_2,45% N_2 and 5% CO_2. Enamel formation was observed in 7 days cultured without IL-1β in histological observation, whereas no enamel formation with IL-1β. On 4 days of organ culture without IL-1β, secretory ameloblasts had already differentiated and Tomes' processes of ameloblasts extended into the mineralized enamel. When cultured in the presence or absence of IL-1β, dentin formation was observed on 2 to 4 days of organ culture. There were denatured cells in tooth germ in histochemical observation. These results demonstrate that IL-1β plays an important role in differentiation of odontoblasts and is not a promotion factor in amelogenesis.
日本大学の論文
2001-12-25