<研究論文>歯胚の成長に及ぼすサイトカインの影響

概要

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The present study investigated the effects of TGF-β1 and IL-1β during mouse tooth development on the basis of organ culture method. The first molars (M 1) from 18-day-old mouse embryos were cultured in vitro by floatation method. This method consisted of using a 24-well culture dish and a culture chamber with a membrane filter. The incubation atmosphere consisted of 50% O_2,45% N_2 and 5% CO_2. Enamel formation was observed by the day 7 without TGF-β1 and IL-1β in histological observation. On day 4 of organ culture, enamel formation was ascertained when cultured in the presence of TGF-β1. Dentin formation was observed by the day 4 without cytokines. On day 2 of culture, dentin formation was shown in the presence of TGF-β1. When cultured in the presence of IL-1β, thin layer dentin formation was recognized by the day 2 culture and there were denatured cells in tooth germ in histochemical observation. But IL-1β stimulates the proliferation of fibroblasts. These results demonstrate that TGF-β plays an important role in differentiation and secretion of ameloblasts as well as in odontogenesis, and that IL-1β has a growth function of tooth germ.
日本大学の論文
2000-12-25