Climbing Rates of Microtubules Propelled by Dynein after Collision with Microfabricated Walls

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概要

• 論文の詳細を見る

• We proposed a method to characterize the effect of micrometer-scale walls on the motion of microtubules propelled by dynein, a motor protein. The walls were made of resist polymers, such as OEBR1000, SAL601, and PMGI, using e-beam lithography. The pattern of the walls was designed to make microtubules collide with the wall perpendicularly and the number of microtubules crossing over the wall was counted from sequential images obtained with a fluorescence microscope. It was found that the wall, which was higher than approximately 800 nm, stops microtubules from crossing over the wall. The wall made of OEBR1000 prevents microtubules from crossing it more effectively than that made of SAL601 and the overhang is also useful for guiding the microtubule motion.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2012-02-25

著者

• Kojima Hiroaki

  Kobe Advanced Ict Research Center National Institute Of Information And Communications Technology

• Oiwa Kazuhiro

  Kobe Adv Ict Res Ctr

• Sakaue Hiroyuki

  Graduate School Of Advanced Science Of Matter Hiroshima University

• Takahagi Takayuki

  Graduate School Of Advanced Science Of Matter Hiroshima University

• Suzuki Hitoshi

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Kojima Hiroaki

  Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe 651-2492, Japan

• Suzuki Hitoshi

  Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashihiroshima, Hiroshima 739-8530, Japan

• Ashikari Norihiko

  Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashihiroshima, Hiroshima 739-8530, Japan

• Shitaka Yuji

  Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe 651-2492, Japan

• Fujita Kosuke

  Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashihiroshima, Hiroshima 739-8530, Japan

• Sakaue Hiroyuki

  Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashihiroshima, Hiroshima 739-8530, Japan

• Takahagi Takayuki

  Graduate School of Advance Science of Matter, Hiroshima University, 1-3 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan

• Oiwa Kazuhiro

  Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe 651-2492, Japan

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