Simple Method of Synthesizing Nickel--Nitrilotriacetic Acid Gold Nanoparticles with a Narrow Size Distribution for Protein Labeling
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概要
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We developed a simple method to synthesize nickel--nitrilotriacetic acid gold nanoparticles (Ni--NTA Au NPs) with a narrow size distribution for site-specific labeling in protein complexes. Au NPs were synthesized by the reduction of HAuCl<sub>4</sub> using trisodium citrate and tannin acid. Then, the nanoparticle surfaces were modified with NTA and subsequent complexation with Ni2+. The mean diameter of the synthesized Ni--NTA Au NPs was 4.3 nm, and the coefficient of variation was 9%. The specific binding of the Ni--NTA Au NPs to polyhistidine-tagged (His-tagged) proteins was determined by transmission electron microscopy using kinesin and the p62 subunit of dynactin. Consequently, our method is useful for analyzing the substructures of protein complexes.
- 2011-09-25
著者
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Kobayashi Takuya
Department Of Pediatrics Urafune Hospital Of Yokohama City University
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Kitai Toshiyuki
Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Watanabe Yuta
Graduate School Of Arts & Sciences The University Of Tokyo
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Toyoshima Yoko
Graduate School Of Arts & Sciences The University Of Tokyo
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Sakaue Hiroyuki
Graduate School Of Advanced Science Of Matter Hiroshima University
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Takahagi Takayuki
Graduate School Of Advanced Science Of Matter Hiroshima University
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Murayama Takashi
Department Of Ergonomics Kyushu Institute Of Design
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Suzuki Hitoshi
Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Murayama Takashi
Department of Pharmacology, Juntendo University School of Medicine, Bunkyo, Tokyo 113-8421, Japan
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Takahagi Takayuki
Graduate School of Advance Science of Matter, Hiroshima University, 1-3 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan
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Toyoshima Yoko
Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo 153-8902, Japan
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Kitai Toshiyuki
Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashihiroshima, Hiroshima 739-8530, Japan
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Kobayashi Takuya
Department of Pharmacology, Juntendo University School of Medicine, Bunkyo, Tokyo 113-8421, Japan
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Watanabe Yuta
Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo 153-8902, Japan
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MURAYAMA Takashi
Department of Biology, Faculty of Science, Chiba University
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