Development of Complementary Metal Oxide Semiconductor Imaging Devices for Detecting Green Fluorescent Protein in the Deep Brain of a Freely Moving Mouse
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概要
- 論文の詳細を見る
We have developed to observe neural activities in the deep brain of a freely moving mouse with green fluorescent protein (GFP). We implanted a dedicated complementary metal oxide semiconductor (CMOS) imaging device into the hippocampus or the basal ganglion of an anesthetized mouse to confirm the effectiveness of the CMOS imaging device for the detection of GFP generated in the deep brain of the anesthetized mouse. Moreover, we conducted an experiment to demonstrate the capability of the CMOS imaging device to detect GFP in the deep brain of a freely-moving mouse. As a result of the in vivo experiments with two methods of GFP expression, we successfully detected the light intensity of GFP in the hippocampus or the basal ganglion of the anesthetized mouse. Furthermore, we demonstrated that the implanted CMOS imaging device operated well in the freely moving mouse after one week from implantation. We demonstrated the basic technology to realize the observation of neural activities in the deep brain of a freely moving mouse.
- 2009-04-25
著者
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Shiosaka Sadao
Graduate School Of Materials Science Nara Institute Of Science And Technology
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Ohta Jun
Graduate School Of Materials Science Nara Institute Of Science And Technology
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Sasagawa Kiyotaka
Graduate School Of Materials Science Nara Institute Of Science And Technology
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Tokuda Takashi
Graduate School Of Materials Science Nara Institute Of Science And Technology
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Tamura Hideki
Graduate Program Of Human Sensing And Functional Sensor Engineering Yamagata University
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Sasagawa Kiyotaka
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Sugiyama Tetsuya
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Tagawa Ayato
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan
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Hatanaka Yumiko
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan
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Ishikawa Yasuyuki
Graduate School of Biological Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Ishikawa Yasuyuki
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Higuchi Asako
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Tagawa Ayato
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Shiosaka Sadao
Graduate School of Biological Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Hatanaka Yumiko
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
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Ohta Jun
Graduate School of Material Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
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