Scanning Force Microscopic Studies of Escherichia coli Ribosomes on Solid Substrate Surface
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概要
- 論文の詳細を見る
Scanning force microscopy (SFM) was used to image ribosomes and ribosomal subunits isolated from Escherichia coli. Topographic images of ribosomal particles adsorbed on a mica surface were obtained directly. At 80 nM, the ribosomes aggregated. At ${\leq}8$ nM, a sparser coverage was achieved, with single ribosomes isolated from one another. Although the obtained height was significantly lower than the diameter measured by X-ray diffraction, the height histogram revealed that the observed ribosomal particles belong to three height distributions: approximately 4, 9 and 11 nm. These three distributions correspond to the 70S ribosome, and the 50S and 30S ribosomal subunits, respectively. This work demonstrates that SFM is useful in the discrimination of very small amounts of ribosomal particles on a solid substrate surface.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2004-07-15
著者
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MATSUURA Toshihiko
Technology Research Laboratory, Shimadzu Corporation
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Kawai Tomoji
The Institute Of Science And Industrial Research (isir-sanken) Osaka University
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Kobayashi Kappei
Technology Research Laboratory Shimadzu Corporation
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Tanaka Hiroyuki
The Institute Of Science And Industrial Research (isir-sanken) Osaka University:jst Presto
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Matsumoto Takuya
The Institute Of Scientific And Industrial Research Osaka University
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Kobayashi Kappei
Technology Research Laboratory, Shimadzu Corporation, 3-9 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan
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Matsumoto Takuya
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
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Tanaka Hiroyuki
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
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Matsuura Toshihiko
Technology Research Laboratory, Shimadzu Corporation, 3-9 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan
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