Electrochemical Detection of Nonlabeled Oligonucleotide DNA Using Biotin-Modified DNA(ss) on a Streptavidin-Modified Gold Electrode
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概要
- 論文の詳細を見る
In the electrochemical detection of nonlabeled DNA, it is important to control the bonding at the interface between the DNA and the electrode. Atomic force microscope (AFM) was taken for the commonly used thiol-modifled DNA on a gold surface. It was found that the coverage of the DNA was very low. On the other hand, a streptavidin-modified gold electrode provided a much better alternative where DNA hybridization resulted in large changes in the electrochemical reaction responses. This work demonstrates that streptavidin-modified gold electrodes could be used in the development of a new electrochemical protocol for the detection of nonlabeled DNA.
- 社団法人日本生物工学会の論文
- 2004-01-25
著者
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KAWAI Tomoji
The Institute of Scientific and Industrial Research, Osaka University
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Kim J
Fed Project Team Samsung Advanced Institute Of Technology
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川合 知二
大阪大学 産業科学研究所
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TANAKA Hiroyuki
The Institute of Scientific and Industrial Research, Osaka University
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Kim Jong
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Kawai Tomoji
The Institute Of Science And Industrial Research Osaka University
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Kanno Takashi
The Institute Of Scientific And Industrial Research Osaka University
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Tabata H
Department Of Bioengineering School Of Engineering University Of Tokyo
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Tabata Hitoshi
The Institute Of Science And Industrial Research Osaka University
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Tabata Hitoshi
The Authors Are With The Institute Of Scientific And Industrial Research Osaka University
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TANAKA Hidekazu
The Institute of Scientific and Industrial Research, Osaka University
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Lee Hea-yeon
The Institute Of Scientific And Industrial Research Osaka University
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Kawai Tomoji
The Institute Of Science And Industrial Research (isir-sanken) Osaka University
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Tanaka H
The Institute Of Scientific And Industrial Research Osaka University
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Kim Jong
Fed Project Team Samsung Advanced Institute Of Technology
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Kim Jong
The Institute Of Scientific And Industrial Research Osaka University
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Park Jong
The Institute Of Scientific And Industrial Research Osaka University
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YAMASAKI RYUJIRO
The Institute of Scientific and Industrial Research, Osaka University
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Yamasaki R
The Institute Of Scientific And Industrial Research Osaka University
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Tanaka Hiroyuki
The Institute Of Science And Industrial Research (isir-sanken) Osaka University:jst Presto
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Tanaka Hidekazu
The Institute Of Scientific And Industrial Research Osaka University
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Lee Hae-Yeon
The Institute of Scientific and Industrial Research, Osaka University
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