Native connection from porcine cardiac muscle.
スポンサーリンク
概要
- 論文の詳細を見る
Native connectin was isolated from porcine cardiac muscle using the method developed for the preparation of native connectin from chicken breast muscle (Kimura et al. (1984) J. Biochem. 96, 1947-1950). It was not necessary to keep cardiac muscle at 0°C before preparation: the proteolysis of α-connectin to β-connectin proceeded during the preparation of myofibrils. Cardiac connectin showed almost the same properties as those of skeletal muscle connectin: mobility in SDS gel electrophoresis, filamentous structure under an electron microscope, circular dichroism spectra, UV absorption spectra, and amino acid composition. Porcine cardiac connectin cross-reacted with antiserum against chicken breast muscle connectin as revealed by an immunoblot method. Immunoelectron microscopical observations revealed an abundance of connectin antigenic sites around the A-I junction area of cardiac myofibrils. Cardiac connectin also interacted with myosin and actin fila-ments at low ionic strengths to form aggregates. The extent of interaction was somewhat weaker in the case of cardiac connectin than skeletal muscle connectin, regardless of the origin of myosin and actin (porcine cardiac and rabbit skeletal muscles). In conclusion, cardiac connectin is very similar, but not identical to skeletal muscle connectin.
- 社団法人 日本生化学会の論文
著者
-
Kimura Sumiko
Department Of Anatomy And Developmental Biology Kyoto University
-
Ohashi Kazuyo
Department Of Biology Faculty Of Science Chiba University
-
Maruyama Koscak
Department Of Biology Faculty Of Science Chiba University
-
Suzuki Tsuneo
Department Of Forensic Medicine Yamagata University School Of Medicine
-
ITOH Yoshiharu
Department of Biology, Faculty of Science, Chiba University
関連論文
- ANOREXIGENIC ACTION OF PACAP MEDIATED BY CRF IN THE GOLDFISH, CARASSIUS AURATUS
- EFFECT OF MELANIN-CONCENTRATING HORMONE ON FOOD INTAKE AND LOCOMOTOR ACTIVITY IN THE GOLDFISH, CARASSIUS AURATUS
- STIMULATORY EFFECT OF ACYL GHRELIN, BUT NOT DES-ACYL GHRELIN, ON LOCOMOTOR ACTIVITY IN THE GOLDFISH
- Inter-Meson Potentials in Dual Ginzburg-Landau Theory : Particles and Fields
- Sensitive determination of methomyl in blood using gas chromatography-mass spectrometry as its oxime tert.-butyldimethylsilyl derivative
- Molecular Cloning of a Partial cDNA Clone Encoding the C Terminal Region of Chicken Breast Muscle Connectin
- ITIH1^* Q0_, A NULL ALLELE OF INTER-ALPHA-TRYPSIN INHIBITOR H1 CAUSED BY DELETION/FRAMESHIFT MUTATION
- CHARACTERIZATION OF THE GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN 45α IN MEDAKA FISH(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- On a Phase with Spontaneously Broken Scale Invariance in Three Dimensional O(N) Models
- A Constraint from the Kallen-Lehmann Representation on the (φ^4)_4 Theory
- LOCALIZATION OF lasp-2 IN CHICKEN PRIMARY CULTURED CELL(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Calcium-Activated Neutral Protease Quickly Converts α-Connectin to β-Connectin in Chicken Breast Muscle Myofibrils : COMMUNICATION : Biochemistry
- Localization of Three Fragments of connectin in Chicken Breast Muscle Sarcomers
- THE PRIMARY STRUCTURE OF CONNECTIN-LIKE ELASTIC PROTEIN OF ANNELID OBLIQUELY STRIATED MUSCLE(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Characterization of Arp2/3 Complex in Chicken Tissues
- A Comment on a New Interpretation of the Chiral Anomaly
- Eight Quark Model with Modified Left-Handed Currents
- THE PRIMARY STRUCTURE OF CHICKEN BREAST MUSCLE CONNECTIN (TITIN)(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- PRIMARY STRUCTURE OF THE I BAND REGION (900 KDA) CONNECTIN/TITIN FROM CHIKEN BREAST MUSCLE(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- A 7.5-kb 3'-Terminal cDNA Sequence of Chicken Skeletal Muscle Nebulin Reveals Its Actin Binding Regions : Cell Biology
- Myofibrils and Meat Texture of Broilers Fed on a Diet of Tochu Leaf Powder
- Localization of Connectin-like Proteins in the Giant Sarcomeres of Barnacle Muscle
- PART II Phenomenological Analyses of High Energy Scattering
- Structure and Mechanical Properties of Diamond-Like Carbon Films Prepared from C2H2 and H2 Mixtures by Pulse Plasma Chemical Vapor Deposition
- Contractility of Myosin B Fibers
- Protruding masticatory (superfast) myosin heads from staggered thick filaments of dog jaw muscle revealed by X-ray diffraction
- 1P225 Passive and active tension generation of striated muscles with reference to the domain structure of their connectin/titin.(8. Muscle contraction and muscle protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Changes in the Actin-Modified ATPase Activity of Myosin during Storage
- Cylinder Protein Isolated from Rat Liver Mitochondria(Biochemistry)
- ANALYSIS OF ACTIN-BINDING PROTEINS IN CHICKEN BRAIN BY 2D-PAGE(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- SEARCHING OF FILAMIN B-BINDING PROTEIN FROM CHICKEN BRAIN(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- K-N Inelastic Processes
- β-Actinin, an Actin-Capping Protein of Striated Muscle
- DIVERSITY OF CONNECTIN (TITIN)-LIKE GIANT PROTEIN IN INVERTEBRATE MUSCLES(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Comparative investigations of human and rat dermatoglyphics: palmar, plantar and digital pads and flexion creases
- A 260-kDa Filamin/ABP-Related Protein in Chicken Gizzard Smooth Muscle Cells Is a New Component of the Dense Plaques and Dense Bodies of Smooth Muscle
- In vitro Dimerization of I-protein, an A-I Junctional Component of Skeletal Muscle Myofibrils : Biochemistry
- Simple Properties of Corrections to the Regge-Pole Amplitudes in Charge and Hypercharge Exchange Reactions
- Monopole Condensation and String Theory of Hadrons(Recent Developments in QCD and Hadron Physics)
- Isolation and Characterization of Sea Sponge Myosin
- avEna 3, A NEW AVIAN HOMOLOG OF Ena(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- CHICKEN ENA FAMILY PROTEINS(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- The New Event in Cosmic Ray Showers and the U(4) Strange Spin Formalism
- Regge Pole Model with Flat (α(t)=0) Trajectories
- A Theory of Primary Vector and Axial-Vector Interaction
- C-TERMINAL REGION OF NEBULIN FROM AMPHIOXUS STRIATED MUSCLE(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Interaction of F-actin, Spectrin and α-Actinin
- 1P235 Single molecule measurement of Elasticity of connectin-like 1200K-protein in obliquely striated muscle of a polychaete (Annelida)(8. Muscle contraction and muscle protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Some Remarks on Null-Plane Quantization
- Chiral Symmetry Breaking and K^0_S→2π Decay
- SINGLE MOLECULE MEASUREMENT OF ELASTICITY OF INVERTEBRATE CONNECTIN(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Direct Visualization of the Effect of Mg^ on the Elongation at the Pointed Ends of Limulus Acrosomal Actin Bundles(COMMUNICATION)(Cell Biology)
- Chiral Invariant Lagrangian with Massive Gauge Fields : Decays of Scalar Mesons and π-π Scattering
- Strange Spin Formalism for Hadrons
- INTERACTION OF CHICKEN DIAPHANOUS PROTEIN WITH ACTIN(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- SEARCHING OF ACTIN-BINDING PROTEINS FROM CHICKEN BRAIN WITH F-ACTIN AFFINITY COLUMN CHROMATOGRAPHY(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Effective Activation of Myosin ATPase by Actin Contained in Myosin Preparations
- Abelian Confinement Mechanism and QCD Vacuum : Particles and Fields
- Dencofinement Transition and Monopoles in T≠0 SU(2) QCD
- Does Muscle β-Actinin Affect the Ouabain Sensitivity of (Na, K)ATPase Activity of Cell Membranes?(Biochemistry)
- Hydroxylation of Protocollagen-^C-proline by a Muscle Extract
- 2P-101 環形動物斜紋筋のコネクチン様4000K蛋白質の部分配列(筋肉(筋蛋白質・収縮),第47回日本生物物理学会年会)
- Relativistically Covariant Formulation in Non-Linear Lagrangian Theories and Factor Ordering Problems
- A Comment on Chiral Invariant Lagrangian and Soft-Pion Theorems
- Effect of pH and KC1 Concentration on the ATP-Actomyosin Interaction
- Primary Vector and Axial-Vector Interaction Model and K_ Decays
- Pathology of Minimal Subtraction Renormalization in the Analytically Regularized 1/N Expansion : Particles and Fields
- A Nontrivial Ultraviolet Fixed Point and Stability of Three-Dimensional O(N) Model with Fermions and Bosons : Particles and Fields
- Monopoles and Quark Confinement
- Gauge Independent Mechanism of Decoupling at Low Energies
- The Thirring Model in the Indefinite-Metric Framework
- K_ Decays and (1,8) + (8,1) Symmetry Breaking
- Confinement Criteria and Compact (QED)_
- Effect of ATP Concentration on the Superprecipitation and Clearing of Actomyosin
- Effect of Chloromercuribenzoate on the Mg-Enhanced ATPase Activity of Actomyosin
- Connectin an Elastic Protein from Myofibrils and Sarcolemma of Frog Skeletal Muscle(短報)
- Theory of a Fusion Field Composed of Free Massless Spinor Fields in Two Dimensions
- An Indefinite-Metric Formulation of a Free Massless Scalar Theory in Two Dimensions
- Color Confinement and Asymptotic Completeness
- Study of η-η′Mixing and SU(4) Quartet Scheme
- Monopole Dynamics and Confinement in SU(2) QCD
- Appearance of Essential Singularity in the Analytically Regularized 1/N Expansion
- 1PS012 環形動物斜紋筋のコネクチン様4000Kタンパク質の47アミノ酸リピート(日本生物物理学会第50回年会(2012年度))
- Molecular shape of .ALPHA.-connectin, an elastic filamentous protein of skeletal muscle.
- Is nebulin the product of Duchenne muscular dystrophy gene?
- Gizzard actin confers a magnesium sensitivity to skeletal muscle myosin at low ionic strengths.
- Characterization of Myosin Isolated from Bodywall Smooth Muscle of the Annelid, Urechis unicinctus.
- 1PS011 軟体動物横紋筋のコネクチン様タンパク質(日本生物物理学会第50回年会(2012年度))
- Infrared Dichroism of an Elastic Portion (1200kDa Fragment) of Connectin.
- Molecular Shape of Dystrophin with Special References to Type VI Collagen.
- Staining of the nemaline rods by fluorescent antibody against Z-protein.
- Gel electrophoretic study of nemaline muscle.
- Furan Derivatives. I. On the Methods of Synthesizing Some Naphtho[1,8-bc]furans
- Molecular shape of dystrophin purified from rabbit skeletal muscle myofibrils.
- Isolation of dystrophin in denatured form from rabbit skeletal muscle myofibrils.
- Native connection from porcine cardiac muscle.
- 2P138 ヤツメウナギのコネクチン様タンパク質(10.筋肉,ポスター,日本生物物理学会年会第51回(2013年度))