ヒト培養皮膚線維芽細胞におけるdihydrotestosterone産生 (5α-reductase activity)

概要

論文の詳細を見る
Testosterone secreted from the testis is converted to dihydrotestosterone (DHT) in target tissues by 5a-reductase and DHT exerts its action on the uncleus. Since skin is one of the target tissues of androgen, we established a system for culturing human skin fibroblasts, and we investigated the conversion of testosterone to DHT in genital and nongenital skin fibroblasts obtained from males as well as from females.<BR>In our system, fibroblasts were incubated with 0.1μM 1, 2-<SUP>3</SUP>H testosterone, and labeled metabolites were separated by thin layer chromatography. DHT formation was linear for 6 hours and the rate of DHT formation correlated with the growth phase of the fibroblasts. In the course of serial subculture, DHT formation did not change between the 3rd and 10th subculture in four cell strains. DHT formation in nongenital skin fibroblasts did not differ significantly between males and females. Fibroblasts obtained from male genital skin produced significantly greater amounts of DHT than those from male or female nongenital skin. Furthermore, DHT formation in genital skin fibroblasts did not differ between normal males and hypogonadal males.<BR>These data suggest that genital skin is the target tissue where testosterone strongly converts to DHT and the difference of male secondary sexual characteristic appearance between males and females may not depend on the sensitivity of the target tissues to androgen but on the secretion rate of androgen.
一般社団法人日本内分泌学会の論文

ヒト培養皮膚線維芽細胞におけるdihydrotestosterone産生 (5α-reductase activity)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク