フローサイトメトリーを用いたDNA/BrdU 2重染色法による1 - hexylcarbamoy 1-5- fluorouracil の抗腫瘍効果と作用機序の解明

概要

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An investigation was carried out to elucidate the mechanism of action of the the oral antitumor agent 1hexylcarbamoy1-5-fluorouracil (HCFU) by determining its effects on the growth and cell cycle of epipharyngeal carcinoma cells (KB cell) by BrdU/DNA double staingusing flow cytometory (FCM). As a result, it was found that HCFU stimulates KB cell in the S phase to proliferate for the first 3 days of trearment in a low concentration (8μg/ml) and caused cell accumulation in the later G2M phase. On the other hand, when administered in the concentration (20μg/ml) that produces a 50 per cent cell kill, as determined from the cell growth curve, HCFU appeared to exhibit a cytocidal effect by blocking cells in S and G2M for the first 3days after exposure. It was revealed by FCM for the first time that HCFU operates by a similar mechanism to that of 5-FU. This method seems to be of significance to therapeutic schemes that take into consideration the mechanism of action of antitumor drugs.
Kinki Brain Tumor Pathology Conferenceの論文