脳腫瘍培養畑胞に対するACNU,MCNUの作用機序の比較検討

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ACNU and MCNU which have been used in general against malignant brain tumor were analized their action mechanism by using of flow cytometry(FCM).<BR>Cell lines of KMU-430 and U-251 drived from maligmant glioma were used. These cells of 1×105 cells/ml were cultured under MEM with 10% FBS and contacted for 24 hours with different concentrations of 1,2,5,10,20,40,80mg/ml of ACNU or MCNU.<BR>Growth curve was obtained from cell growth in vitro. FCM analysis was applied for the cell viability using of fluorescein diacetate staining and DNA-histogram using of propidium iodide.<BR>20-40mg/ml of ACNU showed cytostatic effect for 24 hours and 80mg/ml showed cytocytal effect in cach cell lines.2-20mg/ml of ACNU and 1-10mg/ml of MCNU affected the viability below 80 after 24hours, but it's viability was gradually increased until 4th days. Over 40mg/ml of ACNU or 20mg/ml of MCNU revealed cytocylal effect after 5th days. DNA-histogram was obtained the S phase accumulation 2-4th days in 5-10mg/ml of ACNU, but ACNU and MCNU basically similar action mechanism of G2 M block in cell cycle.<BR>Antineopastic effect was different between ACNU and MCNU but action mechanism was simillar patterns of G2M block in cell cycle with use of FCM.
Kinki Brain Tumor Pathology Conferenceの論文