Mechanism of anticancer drug action in head and neck cancer demonstrated by flow cytometry. First report: Analysis of mechanism of cisplatin action by three-dimensional color display.:-First Report: Analysis of Mechanism of Cisplatin Action by Three-dimen

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For the treatment of tumors in the head and neck region, surgery, chemotherapy and radiotherapy can be used. To obtain better chemotherapeutic results, it is important to know the antineoplastic mechanism and/or how to combine the drugs that are available at present. The effect of an anticancer drug, cisplatin, on cell dynamics was display two-dimensionally by flow cytometry with FITC-label bromodioxyuridine and propidium iodide, converted to a three-dimensional color display by computer processing. This makes it possible to demonstrate the cell dynamics changes better visually. Simultaneously, using fluorescein diacetate, the viability was measured to study the cytocidal effect. Three dimensional histogram by administration of cisplatin in concentration of 0.5μg/ml showed the cell accumulation in S phase on 1st day and then represented the block of G2M phase on 5th day.Newly deviced our methods could be applied to obtain the cell kinetics in vitro and also to solve the mechanism of other antineoplastic agents.
耳鼻咽喉科臨床学会の論文