A Rapid Loop-Mediated Isothermal Amplification Assay Targeting hspX for the Detection of Mycobacterium tuberculosis Complex
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概要
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A rapid, simple, and low-cost diagnostic tool for tuberculosis (TB) detection is urgently needed in countries with a high TB burden. Here, we report a novel loop-mediated isothermal amplification (LAMP) assay targeting the hspX gene for the rapid detection of Mycobacterium tuberculosis, M. bovis, M. africanum, and M. microti. The specificity of this assay was evaluated using 4 reference strains of Mycobacterium tuberculosis complex (MTC), 22 species of non-tuberculous mycobacteria (NTM), 7 non-mycobacterial species, and 50 clinical M. tuberculosis isolates. All the reference MTC strains and M. tuberculosis clinical isolates were successfully detected by this method, and there were no false-positive results with NTM or non-mycobacterial species, which demonstrates the high specificity of this assay for MTC. The detection limit was 10 copies of MTC genome within 27 min, and the detection speed of this assay was higher than that of any other isothermal methods reported so far. Because of its speed, simplicity, sensitivity, specificity, and inexpensiveness, the TB hspX LAMP assay is a potential gene diagnostic method for TB detection in developing countries with a high TB burden.
著者
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Tamaru Aki
Department Of Microbiology Osaka Prefectural Institute Of Public Health
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Sugawara Isamu
Department Of Molecular Pathology Reserch Institute Of Tuberculslosis
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Kimura Akio
Department Of Anesthesiology And Intensive Care Kyoto Prefectural University Of Medicine
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Bi Aixiao
Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine
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Suzuki Yasuhiko
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
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Nakajima Chie
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
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Fukushima Yukari
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
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Kawahara Ryuji
Department of Infectious Diseases, Osaka Prefectural Institute of Public Health
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Hu Zhongyi
Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine
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Sugawara Isamu
Department of Mycobacterium and Research, Research Institute of Tuberculosis
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Tamaru Aki
Department of Infectious Diseases, Osaka Prefectural Institute of Public Health
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