Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification
スポンサーリンク
概要
- 論文の詳細を見る
Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically feasible method for rapid and accurate identification of M. tuberculosis complex.
著者
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Sawanpanyalert Pathom
National Institute Of Health Department Of Medical Sciences Ministry Of Public Health
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Suzuki Yasuhiko
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
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Nakajima Chie
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control
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Sawanpanyalert Pathom
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
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Rudeeaneksin Janisara
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
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Bunchoo Supranee
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
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Srisungngam Sopa
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
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Chamnangrom Sawet
Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health
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Kamolwat Atipa
Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health
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Thanasripakdeekul Porntip
Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health
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Taniguchi Tooru
Research Collaboration Center for Emerging and Re-emerging Diseases, Osaka University
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Phetsuksiri Benjawan
National Institute of Health, Department of Medical Sciences, Ministry of Public Health
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