ヒト膵癌の膵液膵組織中の糖タンパクについて
スポンサーリンク
概要
- 論文の詳細を見る
Recently, several kinds of antibodies against tumor specific antigens, including AFP and CEA, have been developed and used in cancer diagnosis. Due to the difficulty of clinical diagnosis of pancreatic cancer in its early stages, many studies to discover pancreatic tumorspecific antigens have been carried out. Since we found that pancreatic fluids obtained from pancreatic cancer patients contained not only free sialic acid at higher concentrations than those of the control, we tried to isolate and characterize glycoproteins from both pancreatic fluid and carcinomic tissue by means of electrophoresis. Both pancreatic fluids from pancreatic cancer patients and from controls were found to contain 8 major proteins on polyacrylamide gel electrophoresis (PAGE), each having the same relative mobility. When PAS-staining was performed after PAGE, 4 PAS-positive bands showing identical mobility were observed in both controls and pancreatic cancer patients. In addition to these 4 glycoproteins, pancreatic fluid from pancreatic cancer patients contained an additional 2 glycoproteins. One glycoprotein, which was found in only pancreatic cancer patients with Rm 0.68 on PAGE, and this was very strongly stained by PAS-reagent. Since the intensity of the purple coloration of each glycoprotein in cancer patients was diminished after neuraminidase treatment, it was indicated that sialic acid content in sugar moiety of the protein was increased in pancreatic cancer. The molecular weight of pancreatic cancer-specific glycoprotein with Rm 0.68 was determined to be approximately 66, 000 dalton by SDS-PAGE. This 66, 000 dalton glycoprotein showed no immunoreactivity with anti-human serum antibody or with POA (pancreatic oncofetal antigen) antibody by immunoelectrophoresis. This glycoprotein was quite different from both AFP and CEA in its malecular structure. The 66, 000 dalton glycoprotein was found in 73% of the pancreatic cacer patients (8 cases in 11) . When glycoproteins were isolated from plasma membrance fractions of pancreatic tissue by LIS-phenol procedure, the 66, 000 dalton glycoprotein found in pancreatic fluid was undetectable on PAGE. On the other hand, the plasma membran contained 72, 000 dalton glycoprotein, which could not be found in control tissue. The characterizatioh of the 72, 000 dalton glycoprotein is no being undertaken.
- 学校法人 昭和大学・昭和医学会の論文
著者
関連論文
- 47 ドプラ断層法による肝細胞癌境界部血流の検討(第36回日本消化器外科学会総会)
- 477 胆道癌の超音波ドプラ断層像の検討(第37回日本消化器外科学会総会)
- 273 肝腫瘍鑑別診断法としてのドプラ断層法の有用性と意義について(第32回日本消化器外科学会総会)
- 示-72 ドップラー断層法の肝腫瘍鑑別診断への応用(第31回日本消化器外科学会総会)
- 158 肝腫瘍におけるドップラー断層超音波検査法の臨床経験(第30回日本消化器外科学会総会)
- 354 ドップラー断層法による肝硬変症の門脈血行動態の観察とその意義(第29回日本消化器外科学会総会)
- (5)胆汁酸動態
- アルコール性肝障害における血漿アミノ酸組成の変動 : 特に Tyrosine の低下について
- ラットの成長に伴う膵外分泌と小腸の negative feedback regulation の発達
- 胆石症における胆汁酸動態
- 重症急性膵炎死因の解析
- 急性肝炎の回復期に発症した急性化膿性閉塞性胆管炎の1例
- 肝硬変における血清胆汁酸の臨床的意義
- ヒト膵癌の膵液膵組織中の糖タンパクについて
- Amylaseに関する研究
- 肝潅流法による胆汁酸の取り込み機構
- 慢性膵炎における膵液hexosamineの臨床的意義
- 胆道系疾患における超音波検査の有用性について
- 日本における人体有鉤嚢虫症(Cysticercus cellulosae hominis)--感染後30年を経過したと考えられる1剖検例と文献的考察(354例)
- 内視鏡的に摘出し得た胆道内回虫症の1例
- Changes of collagen and non-collagen protein metabolisms in rat acute pancreatitis induced by ethionine.
- Collagen metabolism in ethionine induced rat chronic pancreatic injury.
- 慢性下痢を主訴とした胆嚢結腸瘻の1例
- Gardner症候群の1例
- 糖尿病患者における胆嚢機能および膵機能について
- 虫垂カルチノイドの1例
- 急性化膿性胆管炎の1例
- 肝硬変難治腹水症および癌性腹水症における濃縮腹水環流装置での治療
- Ampicillin投与による出血性腸炎の1例
- Systemic lupus erythematosus(SLE)にthrombotic thrombocytopenic purpura(TTP)を合併した1症例
- 成人にみられた先天性総胆管拡張症に合併した胆嚢良性腫瘍の1例
- 膵石症4例
- 当教室〔昭和大学第2内科学教室〕における膵疾患,肝腫瘍の腹部超音波検査
- 糖負荷における血中amylaseの変動について
- 閉塞性黄疸の超音波診断
- 広範な肺内転移をきたした細気管支肺胞上皮癌の1例
- 好酸球増多症を伴った膵炎(膵障害)の3症例
- 肝硬変(肝生検病理シリ-ズ-4-)
- 肝疾患における血清amylaseの臨床的検討
- タイトル無し
- Clinical evaluation of per-rectal portal scintigraphy in patients with liver cirrhosis.
- Serum and ascitic phospholipase A2 activities and their heat stabilities in taurocholate induced rat acute pancreatitis.
- タイトル無し
- タイトル無し