p-azophenobarbital homologous albuminに対するウサギの免疫応答〔英文〕

概要

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Antibody production in the rabbit immunized with rabbit serum albumin conjugated with various number of p-aminophenobarbital by diazocoupling was examined. Anti-hapten antibodies were titrated by passive hemagglutination (PHA) using formalized rabbit erythrocytes (FRE) conjugated with Phenobarbital. Anti-immunogen antibodies were titrated by PHA using immunogen-coated FRE. Antibodies against new antigenic determinants (NAD) generated on the homologous albumin by diazocoupling were evaluated by the hapten inhibition of antibodies in PHA using immunogen-coated FRE. Anti-phenobarbital antibodies were detected in all of the sera obtained at 6 th week after single immunization of antigens in Freund's complete adjuvant. The kinetics of the anti-hapten antibody production varied depending upon the density of the conjugating hapten. Anti-NAD antibodies were remarkably detected in the groups of rabbits immunized with antigens of low papten density (1-2 of hapten/carrier molecule) and were hardly detected in rabbits immunized with highly conjugated antigen (20 of hapten/carrier molecule) .