吸入感染法によるモルモットに対するBCG免疫度の測定法に関する実験的研究(補遺)

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It has already been reported by the same authors that the airborne infection method was useful to measure the degree of immunity induced by BCG vaccination in the experimental tuberculosis of guinea pigs. As the interval between BCG vaccination and the challenge infection seems to be an influencial factor on the evaluation of the manifestation of immunity, a supplemental experimental was carried out on this point comparing the airborne and subcutaneous infection methods. The design of experiment is shown in Fig.1.10-3 mg or 10-1 mg of BCG was inoculated subcutaneously into the left abdomen of guinea pigs. The number of viable unit of the employed BCG was 2.4 × 107 per mg. Virulent tubercle bacilli of the human type (Kurono strain) grown 7 days in Dubos media were employed as the source of the challenge inoculum. The co ncentration of bacillary suspension was adjusted to O.02 mg/ml by photocolorimeter (Hitachi EPO), and then it was passed through a filter paper of Toyo Roshi No.101 to get the filtrate containing only single bacilli. For the airborne infection, guinea pigs were exposed to this filtrate for 30 minutes. For the subcutaneous infection, O.5 ml of the same filtrate was inoculated subcutaneously into the right abdomen. Fig s.2 and 3 show the macroscopic findings of the airborne infection group and of the subcutaneous infection group, respectively. In the case of the airborne challenge conducted at 3 week interval after BCG vaccination, the degree of immunity is demonstrated by the pathological changes of the extra-pulmonary organs such as the spleen and the liver (Fig.2 A). If the airborne challenge is made at 6 week interval after BCG vaccination, the degree of immunity is demonstrated by the changes of the lung (Fig.2 B). In the case of subcutaneous challenge, the existence of BCG immunity is clearly shown, though no significant difference in the degree of immunity is found according to the dose of BCG (Fig.3). The result of viable bacterial count of organs is shown in Table 1. In the case of the airborne challenge conducted at 3 week interval after BCG vaccination, viable bacterial count in the lung and the spleen is much smaller than that of the non-vaccinated group. Comparing the viable unit in the spleen, the group vaccinated with 10--1 mg BCG showed smaller figures than the group vaccinated with 10-1 mg BCG. In the case of airborne challenge made a t 6 week interval after BCG vaccination, the viable bacterial count in the lung and the spleen is less in the group vaccinated with 10-1, mg BCG than in the group vaccinated with 10-3 mg BCG. On the other hand, in the case of the subcutan e ous infection conducted at 3 week interval after the vaccination by 10-1 mg BCG, the multiplication of challenged bacilli is markedly inhibited, while this inhibition is seen only slightly in the group vaccinated with 10-3mg BCG. If the challenge is carried out subcutaneously at 6 week interval after BCG vaccination, the multiplication of challenged bacilli is markedly inhibited even in the group iccinated with 10-3 mg BCG.
日本結核病学会の論文

吸入感染法によるモルモットに対するBCG免疫度の測定法に関する実験的研究(補遺)

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