マウスの凍結全身切片標本による14C-INHの体内分布の観察全身マクロオートラジォグラフィーとフィルムシンチレーションカウンターによる追跡

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Changes in the distribution of 14C-labelled INH in mouse body with the lapse of time was studied by combining the macroautoradiography (ARG) of whole body section of frozen mice and measuring radioactivity with the film scintillation counter. Mice were injected subcutaneously 20μCi of 14C-INH (carbonyl 14C, specific radioactivity 9.6mCi/mM), and at 1, 6 and 24 hours after injection each one mouse was killed and rapidly frozen in aceton-dry ice (-78°C). Then, the whole body of each mouse was sectioned in the thickness of about 20μm by Leitz 1300microtome in cryostat at the temperature of -20°C. ARG of the whole body section was ca r ried out by the contact method using Sakura industrial X-ray film. After four weeks exposure X-ray film was developped and fixed. Distribution of 14C in the various organs was compared by the density of film. As seen in photo.1, at one hour 14C-INH was distributed in the whole body including the subcutaneous tissue, muscles and markedly in the intestines, stomach, liver and brain. After one hour 14C-INH was discharged so rapidly that at 6 hours 14C was ha r dly proved in the subcutaneous tissue and muscles but remained in the lung, liver, stomach, brain and kidney, and it was also transmitted to urine bladder. It is thought that 14C disappears from the circulating blood at six hours, as it cannot be found already in the heart. At twenty-four hours significant photodensity was seen only in the liver, and its density was similar to that at six hours. The radioactive substance in the liver at 24 hours is not probably INH itself, but some labelled metabolites of INH retained in the liver. Radioactivities of the whole body section and of each organ per 1 cm14 by film scintillation counter are shown in Fig.1, and the disintegrated rate calibrated from the count rate coincides well with that of ARG. By combining the both methods, the more reliable informations on the distribution of labelled chemicals are obtained.
日本結核病学会の論文

マウスの凍結全身切片標本による<SUP>14</SUP>C-INHの体内分布の観察全身マクロオートラジォグラフィーとフィルムシンチレーションカウンターによる追跡

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