吸入感染法によるマウスのBCG免疫度の測定法に関する実験的研究

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The authors reported previously that the airborne infection method was useful to measure the degree of BCG-immunity in guinea pigs. There are already some reports on tuberculosis immunity of mice using the airborne infection method, but we have no fundamental experiment to prove this method to be useful as a method for challenge. The authors studied whether or not the airborne challenge method was useful to measure the degree of tuberculosis immunity in mice as well as in guinea pigs. The experiments were carried out comparing the airborne and intravenous infection methods. 1. Materials and methods The experimental design is shown in Table 1. Ten male mice of DD strain were employed in each group.0.1 mg or 0.001 mg of dried BCG was inoculated into tail vein of a mouse of the vaccination groups. The number of viable units of the employed BCG was 8.5×104/mg. Virulent tubercle bacilli of human type (Kurono strain) grown 7 days in Dubos media were employed as the source of challenge inoculum. Mice were challenged with a large size or a small size of virulent bacilli for both the airborne infection and the intravenous one. For the airborne infection with a large size of virulent bacilli, the number o f virulent becilli fixed in lungs was 45 per mouse and in the case of small size the number of bacilli fixed in lungs was 5 per mouse. In the case of the intra v enous challenge with a large size, bacilli of 2.2×105 were inoculated into a mouse, and for the groups to be challenged with a small size, a mouse was inoculated with the bacilli of 2.2×103. 2. Results and comment Table 2 shows th e results of the airborne challenge, and Table 3 shows those of the intravenous challenge. When mice were challenged by the airborne route, the degree of tuberculosis immunity by BCG was observed to correspond with the dosis of vaccination both in challenge by large size and in challenge by small size. On the contrary, by the intravenous challenge the immunizing effect of vaccination with 0.001 mg of BCG was not proved. As shown in Table 4, not as mall number of BCG survives particularly in the spleen even at the fourth week after the intravenous inoculation. When mice are challenged intravenously at such a time, some undesirable reaction as tuberculin shock might appear among the host, challenge bacilli and BCG. In the case of ai r b orne infection, there is hardly any possibility of such undesirable reaction because both the surviving number of BCG in the lungs and the number of inhaled virulent bacilli will be relatively small. From the above-mentioned points, it is considered that the challenge method by airborne route is useful to decide the degree of BCG immunity.
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吸入感染法によるマウスのBCG免疫度の測定法に関する実験的研究

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