Vibrio parahaemolyticus K-株O抗原脂質多糖体の分離と毒性について

概要

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Lipopolysaccharide (LPS) was extracted from the K minus strain of Vibrio parahaemolyticus with 45% phenol containing 3% NaCl. The resulting crude extract (Fr. I) was further fractionated by centrifugation at 100, 000×g. The precipitated fraction (Fr. II) showed high precipitin activity against O antiserum. Lethality for mice was observed in the Fr. II, but not in the supernatant fraction. The LPS (Fr. II) was hydrolyzed with 1% AcOH at 100° for 1 or 2 hours. The non-dialyzable fraction of the hydrolyzed LPS obtained in a cellophan bag indicated much lower precipitin activity than that of intact LPS.These observations appeared to be the result of liberation of an acid-labile, immunologically active portion from the LPS.Mice injected with the Fr. II died, showing typical endotoxic symptoms and diarrhea.
日本細菌学会の論文