プロテアーゼ活性の測定に関するカゼイン寒天プレート法の応用

概要

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Casein agar plate method for protease assay was developed by the method of Cheeseman with a slight modification. With this modified method, 1μg-1 mg/10μl or 10μg-1 mg/0.1 ml of trypsin was successfully determined and the sensitivity of the method was about 10 times higher than that of the original method. This is one of the simple method for the determination of neutral and alkaline protease activity and was found to be applicable to the field of biochemical study. This method is shown to be very suitable for the measurement of activity change observed when protease is treated with various inhibitors and antiserum, respectively. Especially, the results of inhibition test with protease inhibitors by this method agreed well with those obtained by the conventional method determining the remaining protease activity after preincubation of the inhibitor with protease solution. In the clinical analysis with experimental animals, the method was successfully applied for the detection of trypsin in fecal specimen and duodenum Juice. Furthermore, it was also useful for check of routine protease assay of each fraction isolated by column chromatography.
社団法人日本薬学会の論文
1988-06-30