真菌の生化学的,免疫化学的研究-3-

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We extracted the crude artigen from C. albicans with KCl solution of 20% concentrations at ordinary temperature (Fraction K) and from 'cell-residue' with trichloroacetic acid (TCA) in cold (Fraction SI), and then subjected to hot-water extraction for 30 min. from TCA-insoluble 'cell-residue'(Fraction SII).<BR>The following results were obtained from these crude extracts;<BR>I) Influence of heating for 1hr. at 100°C upon serological activity of SI were not observed, but K was slightly effected upon precipitin test, and the change of antigenic titre of K produced by heat treatment was observed in small quantity of decrease of its activity. When candida. anti-serum was selectively absorbed with the fraction K, it was observed that the precipitin reaction of SI was more largely effected than at SII.<BR>II) Results obtained by sephadex fractionation of these crude antigens showed severally the presence of serological active fraction which composed on 2-4 sugars, and these active fraction contained a few glycoprotein which shows similarity on paperelectrophoretic pattern.
日本細菌学会の論文