Aspergillus fumigatus毒素の研究(第2報) : 粗毒素画分中の酸性およびアルカリ性ホスファターゼについて

概要

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Characteristics of the acid and alkaline phosphomonoesterase in crude toxic fraction of Aspergillus fumigatus were investigated and the following results were obtained. 1) Both phosphatase in the crude toxin have not direct participation in activities of lethality, hemolysis and dermonecrosis. Identies between lethality and hemolysis were not observed by inhibition of toxic activities with Hg^<2+> and HCHO. 2) The optimum pH and temperature of the acid phosphatase activity are at pH 4.5 to 4.7 and 50° to 55°. The enzymes are stable between pH 2.8 and 6.7,and below 45°. It was found that the enzymes have abilities to hydrolyze p-nitrophenyl phosphate, pyrophosphate, phenyl phosphate and glucose 6-phosphate, but not to glucose 1-phosphate. MoO_4^<2-> and WO_4^<2-> are effective as inhibitors, whereas Fe^<2+> acted as activator. 3) The optimum pH and temperature of the alkaline phosphatase activity are between pH 10.0 to 10.3 and 50° to 55°, respectively. The enzymes are found to be able to hydrolyze phenyl phosphate, p-nitrophenyl phosphate, glucose 6-phosphate, but pyrophosphate was weakly hydrolyzed. The enzymes were inhibited by I_2,PO_4^<2-> and divalent metal ions such as Cu^<2+> and Pb^<2+>, however Na^+ and K^+ activated them. 4) The acid and alkaline phosphatase of Aspergillus fumigatus showed immunologically different phosphatase activities, respectively.
公益社団法人日本薬学会の論文
1970-08-25