真菌の生化学的免疫化学的研究(第10報) : 酵母glucanの分離と抗原活性について

概要

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Acetone dried cells of Saccharomyces cerevisiae were treated with 45% phenol and further with autoclaving at 135° in water, and then the cell residue was extracted with 3% NaOH under N2 gas at 70° for 40 minute. An alkali-soluble glucan was precipitated from the extracts in a cellophane bag which was dialyzed against running tap water. No nitrogen and phosphorus was found in the gelatinous solid glucan. On the other hand, Fehling-Cu++ solution was added in the above mentioned hot-water extracts, and after removing mannan-Cu complex non-Cu polysaccharide (Fr I, II) was fractionated with DEAE-Sephadex column chromatography. Sugar components of the Fr I and II contained mannose and glucose, and a large quantity of glycogen was estimated in the Fr I but not in the Fr II. Fr-G resulted from dilute HOAc treatment of alkali-soluble glucan contained glucose and a trace of mannose as sugar components.Anti-whole cells serum of S. cerevisiae which was absorbed with a complete purified mannan showed extremely weak precipitin titers (×200∼400) against these glucan fractions. Therefore, it appears that the antigenicity of glucan which located on internal wall of the whole cells may be almost neglected in case of agglutination test.
日本細菌学会の論文