Variation of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 levels in human plasma obtained from 758 Japanese healthy subjects.
スポンサーリンク
概要
- 論文の詳細を見る
Plasma levels of 25-hydroxyvitamin D3 (25-OH-D3) and 25-hydroxyvitamin D2 (25-OH-D2) in 758 Japanese healthy subjects (most of them adults) were determined by a high-performance liquid chromatographic method previously reported (6) and the following results were obtained: 1) The mean and standard deviation (M±SD) of the assayed values of 25-OH-D (sum of 25-OH-D3 and 25-OH-D2) was 23.8±10.1 ng/ml. 2) 25-OH-D3 was detected in all the samples and the M±SD was 23.0±10.1 ng/ml. The plasma levels clearly showed the seasonal variation that the levels in summer were significantly higher than those in winter. Moreover, the plasma levels were significantly correlated with the amounts of UV light in solar radiation. These results strongly suggested that 25-OH-D3 in plasma mainly originated from endogenous vitamin D3 formed by photo-conversion of 7-dehydrocholesterol in skin. 3) 25-OH-D2 was detected only in 18.3% of the plasma samples and the M±SD in the detected samples was 4.4±2.9 ng/ml which was much lower than those of 25-OH-D3. The results suggested that few healthy Japanese are taking daily exogenous vitamin D2 from multivitamin preparations or others. 4) The M±SD values of 25-OH-D3 plasma levels in men and women were 26.2±10.4 and 19.3±8.0 ng/ml, respectively. The formers were significantly higher than the latters. The results were thought to be due to the reason that men might be outdoors for longer periods than women. 5) When age variation of plasma 25-OH-D3 levels was examined, the levels in the twenties were significantly lower than the other generations. This was confirmed to be due to the low values observed in the female twenties group, but the detailed reason is unclear at the present time. 6) When 4 healthy volunteers were orally administered 400 I.U./day of vitamin D2 every day for 8 weeks, maximum levels (average: 11.5 ng/ml) were observed at the 8 weeks and the levels gradually decreased after stopping the administration. The results suggested that the half life of 25-OH-D2 in plasma might be 4-5 weeks.
- 財団法人 学会誌刊行センターの論文
著者
-
小林 正
Department Of Biochemistry Kobe Gakuin University
-
松尾 保
Department of Pediatrics Kobe University School of Medicine
-
岡野 登志夫
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
黒田 英造
Department of Pediatrics, Kobe University School of Medicine
-
児玉 荘一
Department of Pediatrics, Kobe University School of Medicine
-
岡田 かおる
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
志田 澄子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
杉ノ原 敏恵
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
中尾 秀人
Department of Pediatrics, Kobe University School of Medicine
-
岡野 登志夫
Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
-
岡野 登志夫
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
小林 正
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
小林 正
Department of Hygienic Scfences, Kobe Women's College of Pharmacy
-
杉ノ原 敏恵
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
関連論文
- Plasma apolipoprotein and lipid profiles in infants in the first year of life.
- Studies on 1α-Hydroxyl Derivatives of Vitamin D_3. I. : Syntheses of 1α-Hydroxyvitamin D_3 and 1α, 25-Dihydroxyvitamin D_3
- Comparison of 25-Hydroxyvitamin D_3 Binding Proteins from Rat Lymph and Plasma
- Existence of Vitamin D_3 and 25-Hydroxyvitamin D_3 in Rat Lympli
- Bioavailability of Calcium from Calcium Carbonate, DL-Calcium Lactate, L-Calcium Lactate and Powdered Oyster Shell Calcium in Vitamin D-Deficient or -Replete Rats
- Purification of Vitamin D Binding Proteins (Group-Specific Components) in Human Plasma Using a Chromatofocusing Method
- Syntheses of 24,25-Dihydroxyvitamin D_2, 24,25-Dihydroxy-22-dehydrovitamin D_3, 25-Hydroxy-24-oxo-22-dehydrovitamin D_3 and 22,24,25-Trihydroxyvitamin D_3(Organic,Chemical)
- SYNTHESIS OF DIASTEREOMERIC 24,25-DIHYDROXYVITAMIN D_2 AND SEPARATION OF ITS (24R)-AND (24S)-ISOMERS
- Plasma Levels of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 in Maternal, Cord and Neonatal Blood
- ASSAY OF 25-HYDROXYVITAMIN D3 IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- IDENTIFICATION AND DETERMINATION OF 25-HYDROXYVITAMIN D3 IN THE BLOOD AND LIVER OF VITAMIN D-DEFICIENT RATS IRRADIATED WITH ULTRAVIOLET LIGHT
- Simplified Assay of Vitamin D2 in Fortified Dried Milk by Using Two Steps of High-Performance Liquid Chromatography
- STUDIES ON THE ULTRAVIOLET IRRADIATION OF PROVITAMIN D AND ITS RELATED COMPOUNDS III. EFFECT OF WAVELENGTH ON THE FOR-MATION OF POTENTIAL VITAMIN D2 IN THE IRRADIATION OF ERGOSTEROL BY MONO-CHROMATIC ULTRAVIOLET RAYS
- IDENTIFICATION OF VITAMIN D3 AND 7-DEHYDROCHOLESTEROL IN COWS MILK BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND THEIR QUANTITATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYI
- GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D IN MULTIVITAMIN PREPARATION
- GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D3 IN TUNA LIVER AND VITAMIN D3 RESIN OILS
- GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D IN MULTIVITAMIN PREPARATIONS CONTAINING EXCESS AMOUNTS OF VITAMIN E
- A RAPID AND PRECISE METHOD FOR THE DETER-MINATION OF VITAMIN D3 IN RAT SKIN BY NIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- Tissue Distribution of 7-Dehydrocholesterol, Vitamin D3 and 25-Hydroxyvitamin D3 in Several Species of Fishes
- QUANTITATIVE ANALYSIS OF CHOLESTEROL IN FOODS BY GAS-LIQUID CHROMATOGRAPHY
- EFFECT OF WAVELENGTH ON THE FORMATION OF 1α-HYDROXYPREVITAMIN D3 IN THE ULTRAVIOLET IRRADIATION OF CHOLESTA-5, 7-DIENE-1α, 3β-DIOL AND USE OF A FILTER SOLUTION IN THE PHOTOCHEMICAL REACTION IN THE SYNTHESIS OF 1 α-HYDROXY-VITAMIN D3
- Photochemical conversion of 7-dehydrocholesterol into vitamin D3 in rat skins.
- In vivo and in vitro conversion of 7-dehydrocholesterol into vitamin D3 in rat skin by ultraviolet ray's irradiation.
- Studies on the biogenesis of vitamin D. I. Identification and determination of 7-dehydrocholesterol in rat skin.
- A method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography.
- Variation of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 levels in human plasma obtained from 758 Japanese healthy subjects.
- Synthesis and biological activity of 5,6-trans-vitamin D3 in anephric rats.
- Isolation and purification of in vivo-generated 25-hydroxyvitamin D2 by preparative high-performance liquid chromatography.
- Studies on the Ultraviolet Irradiation of Provitamin D and Its Related Compounds:II. Determination of Potential Vitamin D2 in Ultraviolet Irradiated Products of Ergosterol by Gas-Liquid Chromatography
- Decomposition of Vitamin D by the Hydroperoxide Formed from 2, 6-Di-tert-butyl-4-methylphenol during Alkali Saponification
- STUDIES ON THE ISOMERIZATION OF VITAMIN D AND RELATED COMPOUNDS WITH ACIDIC REAGENTS:I. A SIMPLE METHOD FOR THE DETERMINA-TION OF VITAMIN D2 ISOMERS IN MIXTURES
- An improved procedure for the isolation of suprasterol2 I and II from a photochemical reaction mixture of ergocalciferol (vitamin D2).
- Lack of evidence for existence of vitamin D and 25-hydroxyvitamin D sulfates in human breast and cow's milk.
- Effect of wavelength on the photochemical reaction of ergocalciferol (vitamin D2) irradiated by monochromatic ultraviolet light.
- STUDIES ON THE ISOMERIZATION OF VITAMIN D AND RELATED COMPOUNDS WITH ACIDIC REAGENTS:II. MECHANISM OF THE ISOMERIZATION OF VITAMIN D2 AND ITS ISOMERS WITH BORON TRIFLUORIDE
- Bone marrow and serum concentrations of 25-hydroxyvitamin D, 24,25-dihydroxyvitamin D, and 1.ALPHA.,25-dihydroxyvitamin D in patients with leukemia and normal subjects.
- High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk.
- Protein-binding properties of 22-oxa-1.ALPHA.,25-dihydroxyvitamin D3, a synthetic analogue of 1.ALPHA.,25-dyhydroxyvitamin D3.
- Change in blood vitamin levels in surgical patients given a multivitamin preparation (NK-041) for total parenteral nutrition.
- Effect of Solvents on the Thermal Isomerization of 1α-Hydroxyprevitamin D3 Diacetate to 1α-Hydroxyvitamin D3 Diacetate
- The physicochemical and immunological characteristics of rat lymph binding protein for 25-hydroxyvitamin D3.
- Changes in the concentrations of vitamin D and its metabolites in the plasma of healthy subjects orally given physiological doses of vitamin D2 by multivitamin or vitamin D preparations.
- High-performance liquid chromatographic determination of vitamin D in foods, feeds and pharmaceuticals by successive use of reversed-phase and straight-phase columns.
- High-performance liquid chromatographic determination of vitamin D3 in fish liver oils and eel body oils.
- Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrhosis.