IDENTIFICATION AND DETERMINATION OF 25-HYDROXYVITAMIN D3 IN THE BLOOD AND LIVER OF VITAMIN D-DEFICIENT RATS IRRADIATED WITH ULTRAVIOLET LIGHT

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概要

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• The intestinal calcium transport activity and serum calcium and phosphorous concentrations of vitamin D-deficient rats were increased by irradiation with an ultraviolet (UV) lamp. The existence of 25-hydroxyvitamin D3 (25-OH-D3) in their bloods and livers was physicochemically confirmed by high-performance liquid chromatography (HPLC), gas-liquid chromatography (GLC) and mass fragmentography, whereas the compound could not be detected in the tissues of nonirradiated rats. The results strongly suggested that vitamin D3 in vivo generated in irradiated rat skin might be normally metabolized and utilized to prevent rickets. The level of 25-OH-D3 in the tissues was determined by a HPLC method.

• 財団法人 学会誌刊行センターの論文

著者

• 小林 正

  Department Of Biochemistry Kobe Gakuin University

• 水野 久美子

  神戸女子薬大 衛生化学

• 岡野 登志夫

  Department of Hygienic Sciences, Kobe Women's College of Pharmacy

• 水野 久美子

  Department of Hygienic Chemistry, Kobe Womens College of Pharmacy

• 水野 久美子

  Department of Hygienic Chemistry, Kobe Women's College of Pharmacy

• 岡野 登志夫

  Department of Hygienic Chemistry, Kobe Women's College of Pharmacy

• 小林 正

  Department of Hygienic Sciences, Kobe Women's College of Pharmacy

• 小林 正

  Department of Hygienic Scfences, Kobe Women's College of Pharmacy

関連論文

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