In vivo and in vitro conversion of 7-dehydrocholesterol into vitamin D3 in rat skin by ultraviolet ray's irradiation.
スポンサーリンク
概要
- 論文の詳細を見る
In order to confirm the photochemical conversion of 7-dehydrocholesterol (7-DHC) into vitamin D3 in rat skin, the following in vitro and in vivo experiments were carried out. In the first (in vitro) experiment, the skin stripped off from a sacrificed normal rat was irradiated with an ultraviolet (UV) lamp for a constant period. In the second (in vivo) experiment, the normal rat, irradiated under the same condition mentioned above, was sacrificed and then the skin was stripped off. Lipids were individually extracted with chloroform-methanol (1:1) from the skin obtained in the two experiments and the solvent was evaporated. The resulting residue was saponified and the unsaponifiable matter extracted with benzene was purified by application to hydroxyalkoxypropyl (HAP) Sephadex column chromatography. The resulting purified vitamin D3 fraction was applied to high-performance liquid chromatography (HPLC) in order to estimate vitamin D3. No peak, aside from that of α-naphthol as an internal standard, was observed in the HPLC chromatogram on the skin obtained from the non-irradiated rat, whereas the peak corresponding to vitamin D3 was observed in each HPLC chromatogram on both the irradiated skin (in vitro experiment) and the skin obtained from the irradiated rat (in vivo experiment). The peaks, confirmed to be due to vitamin D3 by the results of co-chromatography, were increased according to the increase of irradiation energy and there were little differences between the corresponding estimated values of vitamin D3 in the two experiments. These results prompted the conclusion that 7-DHC in rat skin was photochemically converted into vitamin D3 by UV irradiation and that the in vivo vonversion mechanism might be the same as the in vitro one.
- 財団法人 学会誌刊行センターの論文
著者
-
小林 正
Department Of Biochemistry Kobe Gakuin University
-
岡野 登志夫
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
康村 満枝
Department of Hygienic Chemistry, Kobe Womens College of Pharmacy
-
水野 久美子
Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
-
岡野 登志夫
Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
-
康村 満枝
Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
-
小林 正
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
小林 正
Department of Hygienic Scfences, Kobe Women's College of Pharmacy
関連論文
- Studies on 1α-Hydroxyl Derivatives of Vitamin D_3. I. : Syntheses of 1α-Hydroxyvitamin D_3 and 1α, 25-Dihydroxyvitamin D_3
- Comparison of 25-Hydroxyvitamin D_3 Binding Proteins from Rat Lymph and Plasma
- Existence of Vitamin D_3 and 25-Hydroxyvitamin D_3 in Rat Lympli
- Bioavailability of Calcium from Calcium Carbonate, DL-Calcium Lactate, L-Calcium Lactate and Powdered Oyster Shell Calcium in Vitamin D-Deficient or -Replete Rats
- Purification of Vitamin D Binding Proteins (Group-Specific Components) in Human Plasma Using a Chromatofocusing Method
- Syntheses of 24,25-Dihydroxyvitamin D_2, 24,25-Dihydroxy-22-dehydrovitamin D_3, 25-Hydroxy-24-oxo-22-dehydrovitamin D_3 and 22,24,25-Trihydroxyvitamin D_3(Organic,Chemical)
- SYNTHESIS OF DIASTEREOMERIC 24,25-DIHYDROXYVITAMIN D_2 AND SEPARATION OF ITS (24R)-AND (24S)-ISOMERS
- Plasma Levels of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 in Maternal, Cord and Neonatal Blood
- ASSAY OF 25-HYDROXYVITAMIN D3 IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- IDENTIFICATION AND DETERMINATION OF 25-HYDROXYVITAMIN D3 IN THE BLOOD AND LIVER OF VITAMIN D-DEFICIENT RATS IRRADIATED WITH ULTRAVIOLET LIGHT
- Simplified Assay of Vitamin D2 in Fortified Dried Milk by Using Two Steps of High-Performance Liquid Chromatography
- STUDIES ON THE ULTRAVIOLET IRRADIATION OF PROVITAMIN D AND ITS RELATED COMPOUNDS III. EFFECT OF WAVELENGTH ON THE FOR-MATION OF POTENTIAL VITAMIN D2 IN THE IRRADIATION OF ERGOSTEROL BY MONO-CHROMATIC ULTRAVIOLET RAYS
- IDENTIFICATION OF VITAMIN D3 AND 7-DEHYDROCHOLESTEROL IN COWS MILK BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND THEIR QUANTITATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYI
- GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D IN MULTIVITAMIN PREPARATION
- GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D3 IN TUNA LIVER AND VITAMIN D3 RESIN OILS
- GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D IN MULTIVITAMIN PREPARATIONS CONTAINING EXCESS AMOUNTS OF VITAMIN E
- A RAPID AND PRECISE METHOD FOR THE DETER-MINATION OF VITAMIN D3 IN RAT SKIN BY NIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- Tissue Distribution of 7-Dehydrocholesterol, Vitamin D3 and 25-Hydroxyvitamin D3 in Several Species of Fishes
- QUANTITATIVE ANALYSIS OF CHOLESTEROL IN FOODS BY GAS-LIQUID CHROMATOGRAPHY
- EFFECT OF WAVELENGTH ON THE FORMATION OF 1α-HYDROXYPREVITAMIN D3 IN THE ULTRAVIOLET IRRADIATION OF CHOLESTA-5, 7-DIENE-1α, 3β-DIOL AND USE OF A FILTER SOLUTION IN THE PHOTOCHEMICAL REACTION IN THE SYNTHESIS OF 1 α-HYDROXY-VITAMIN D3
- Photochemical conversion of 7-dehydrocholesterol into vitamin D3 in rat skins.
- In vivo and in vitro conversion of 7-dehydrocholesterol into vitamin D3 in rat skin by ultraviolet ray's irradiation.
- Studies on the biogenesis of vitamin D. I. Identification and determination of 7-dehydrocholesterol in rat skin.
- A method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography.
- Variation of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 levels in human plasma obtained from 758 Japanese healthy subjects.
- Synthesis and biological activity of 5,6-trans-vitamin D3 in anephric rats.
- Isolation and purification of in vivo-generated 25-hydroxyvitamin D2 by preparative high-performance liquid chromatography.
- Studies on the Ultraviolet Irradiation of Provitamin D and Its Related Compounds:II. Determination of Potential Vitamin D2 in Ultraviolet Irradiated Products of Ergosterol by Gas-Liquid Chromatography
- Decomposition of Vitamin D by the Hydroperoxide Formed from 2, 6-Di-tert-butyl-4-methylphenol during Alkali Saponification
- STUDIES ON THE ISOMERIZATION OF VITAMIN D AND RELATED COMPOUNDS WITH ACIDIC REAGENTS:I. A SIMPLE METHOD FOR THE DETERMINA-TION OF VITAMIN D2 ISOMERS IN MIXTURES
- An improved procedure for the isolation of suprasterol2 I and II from a photochemical reaction mixture of ergocalciferol (vitamin D2).
- Lack of evidence for existence of vitamin D and 25-hydroxyvitamin D sulfates in human breast and cow's milk.
- Effect of wavelength on the photochemical reaction of ergocalciferol (vitamin D2) irradiated by monochromatic ultraviolet light.
- STUDIES ON THE ISOMERIZATION OF VITAMIN D AND RELATED COMPOUNDS WITH ACIDIC REAGENTS:II. MECHANISM OF THE ISOMERIZATION OF VITAMIN D2 AND ITS ISOMERS WITH BORON TRIFLUORIDE
- Bone marrow and serum concentrations of 25-hydroxyvitamin D, 24,25-dihydroxyvitamin D, and 1.ALPHA.,25-dihydroxyvitamin D in patients with leukemia and normal subjects.
- High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk.
- Protein-binding properties of 22-oxa-1.ALPHA.,25-dihydroxyvitamin D3, a synthetic analogue of 1.ALPHA.,25-dyhydroxyvitamin D3.
- Change in blood vitamin levels in surgical patients given a multivitamin preparation (NK-041) for total parenteral nutrition.
- Effect of Solvents on the Thermal Isomerization of 1α-Hydroxyprevitamin D3 Diacetate to 1α-Hydroxyvitamin D3 Diacetate
- The physicochemical and immunological characteristics of rat lymph binding protein for 25-hydroxyvitamin D3.
- Changes in the concentrations of vitamin D and its metabolites in the plasma of healthy subjects orally given physiological doses of vitamin D2 by multivitamin or vitamin D preparations.
- High-performance liquid chromatographic determination of vitamin D in foods, feeds and pharmaceuticals by successive use of reversed-phase and straight-phase columns.
- High-performance liquid chromatographic determination of vitamin D3 in fish liver oils and eel body oils.
- Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrhosis.