STUDIES ON THE ISOMERIZATION OF VITAMIN D AND RELATED COMPOUNDS WITH ACIDIC REAGENTS:I. A SIMPLE METHOD FOR THE DETERMINA-TION OF VITAMIN D2 ISOMERS IN MIXTURES

概要

論文の詳細を見る
The determination of vitamin D2 isomers in mixtures was investigated in order to use in kinetic experiments on their isomerization with acidic reagents. Vitamin D2, pre-D2, 5, 6-trans-D2, iso-D2, T2 and iso-T2, thought to exist in the reaction mixtures, were chosen as standard compounds of vitamin D2 isomers. When a mixture of the standard compounds was ap-plied to the GLC using OV-17 as a stationary phase after trimethylsily-lation, the separation of vitamin D2 from pre-D2 and of T2 from iso-T2 were impossible while the two groups and the other compounds were separated one another (Both vitamin D2 and pre-D2 were quantitatively isomerized into the thermal cyclized compounds "pyro- and isopyro-D2" in the same manner.). Therefore, it was possible to determine the compounds by the GLC when the coexistences of vitamin D2 with pre-D2 and of T2 with iso-T2 were simultaneously unidentified, whereas pre-treatment was thought necessary to separate them when their coexistence was identified. When a mixture of the standard compounds was applied to the TLC using Kieselgel GF254 as an adsorbent and benzene-acetone (95:5) as a developing solvent, vitamin D2 from pre-D2 and T2 from iso-T2 were separated each other. From these results, a method based on the TLC and GLC was proposed for the determination of vitamin D2 isomers in mixtures.
財団法人学会誌刊行センターの論文