GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF VITAMIN D3 IN TUNA LIVER AND VITAMIN D3 RESIN OILS

概要

論文の詳細を見る
Gas-liquid chromatographic (GLC) determination of vitamin D3 in tuna liver and vitamin D3 resin oils was investigated and a routine method slightly modified from the previously reported methods (1, 2) was established. Since both tuna liver and vitamin D3 resin oils contained large amounts of sterols, digitonin-Celite column chromato graphy according to SHEPPARD et al. (4) was used to remove the sterol influence from the unsaponifiable matters of the oils. After collecting the eluate and evaporating the solvent, the residue was subjected to thin-layer chromatography (TLC) using Kieselgel GF254 as an adsorbent and a mix ture of n-hexane-ethyl acetate (4:1) as a developing solvent. The scraped zones corresponding to vitamin D3 and pre-D3 were trimethylsilylated and then applied to the GLC using 1.5% OV-17 packed on Shimalite W (80 100 mesh) as a stationary phase. Trimethylsilylation of the gas chro matograms was an essential procedure, because the peaks of unknown substances in tuna liver oils and lumisterol in vitamin D3 resin oils could not be separated from the peak of pyro-D3 without trimethylsilylation. When the proposed method was applied to the samples, satisfactory results were obtained.
財団法人学会誌刊行センターの論文