Existence of Vitamin D_3 and 25-Hydroxyvitamin D_3 in Rat Lympli
スポンサーリンク
概要
- 論文の詳細を見る
The transport forms of vitamin D_3 and 25-hydroxyvitamin D_3 [25 (OH) D_3] in lymph were studied in rats with thoracic duct cannulas. Under in vitro and in vivo conditions, the majority of vitamin D_3 recovered from lymph was found in the chylomicrons and other lipoproteins fractions. On the other hand, the major fraction containing 25 (OH) D_3 corresponded to the albumin and α-globulin fractions. In order to confirm the presence of binding protein having affinity to 25 (OH) D_3 in lymph, the binding protein was isolated and purified by procedures of gel filtration, affinity and ion exchange chromatography from the incubated mixture of thoracic duct lymph with 25 (OH) D_3. The purified binding protein showed a single band of protein on disc and sodium dodecyl sulfate-disc gel electrophoreses. Furthermore, the protein also gave a single peak on highperformance liquid chromatography (HPLC) using a gel permeation column. The molecular weight of the protein was calculated from the results of HPLC as 57000,a value which is apparently different from that of lymph albumin. These results suggest that vitamin D_3 and 25 (OH) D_3 are mainly transported from the intestine to the liver via the lymph duct by different carrier mediators as lipoprotein complex and protein-bound forms, respectively, in rats.
- 公益社団法人日本薬学会の論文
- 1983-09-25
著者
-
村井 純子
神戸女子薬大
-
岡野 登志夫
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
増田 園子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
伊志領 正子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
村井 純子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
山本 由佳子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
伊志領 正子
Department Of Hygienic Sciences Kobe Women's College Of Pharmacy
-
山本 由佳子
神戸女子薬大
-
岡野 登志夫
Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
-
増田 園子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
村井 純子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
-
山本 由佳子
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
関連論文
- Comparison of 25-Hydroxyvitamin D_3 Binding Proteins from Rat Lymph and Plasma
- Existence of Vitamin D_3 and 25-Hydroxyvitamin D_3 in Rat Lympli
- 1-II-10 ラットリンパ及び血漿から精製された25-OH-D Binding Protein の化学的諸性質について : 第35回大会一般研究発表要旨 : 日本ビタミン学会
- 6.ビタミンD並びに25-OH-Dのリンパ管内輸送 : 第200回会議研究発表要旨 : 脂溶性ビタミン総合研究委員会
- Bioavailability of Calcium from Calcium Carbonate, DL-Calcium Lactate, L-Calcium Lactate and Powdered Oyster Shell Calcium in Vitamin D-Deficient or -Replete Rats
- Purification of Vitamin D Binding Proteins (Group-Specific Components) in Human Plasma Using a Chromatofocusing Method
- Syntheses of 24,25-Dihydroxyvitamin D_2, 24,25-Dihydroxy-22-dehydrovitamin D_3, 25-Hydroxy-24-oxo-22-dehydrovitamin D_3 and 22,24,25-Trihydroxyvitamin D_3(Organic,Chemical)
- SYNTHESIS OF DIASTEREOMERIC 24,25-DIHYDROXYVITAMIN D_2 AND SEPARATION OF ITS (24R)-AND (24S)-ISOMERS
- Plasma Levels of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 in Maternal, Cord and Neonatal Blood
- ASSAY OF 25-HYDROXYVITAMIN D3 IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- IDENTIFICATION AND DETERMINATION OF 25-HYDROXYVITAMIN D3 IN THE BLOOD AND LIVER OF VITAMIN D-DEFICIENT RATS IRRADIATED WITH ULTRAVIOLET LIGHT
- Simplified Assay of Vitamin D2 in Fortified Dried Milk by Using Two Steps of High-Performance Liquid Chromatography
- A RAPID AND PRECISE METHOD FOR THE DETER-MINATION OF VITAMIN D3 IN RAT SKIN BY NIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- Tissue Distribution of 7-Dehydrocholesterol, Vitamin D3 and 25-Hydroxyvitamin D3 in Several Species of Fishes
- Photochemical conversion of 7-dehydrocholesterol into vitamin D3 in rat skins.
- In vivo and in vitro conversion of 7-dehydrocholesterol into vitamin D3 in rat skin by ultraviolet ray's irradiation.
- Studies on the biogenesis of vitamin D. I. Identification and determination of 7-dehydrocholesterol in rat skin.
- A method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography.
- Variation of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 levels in human plasma obtained from 758 Japanese healthy subjects.
- Isolation and purification of in vivo-generated 25-hydroxyvitamin D2 by preparative high-performance liquid chromatography.
- Lack of evidence for existence of vitamin D and 25-hydroxyvitamin D sulfates in human breast and cow's milk.
- Bone marrow and serum concentrations of 25-hydroxyvitamin D, 24,25-dihydroxyvitamin D, and 1.ALPHA.,25-dihydroxyvitamin D in patients with leukemia and normal subjects.
- High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk.
- Protein-binding properties of 22-oxa-1.ALPHA.,25-dihydroxyvitamin D3, a synthetic analogue of 1.ALPHA.,25-dyhydroxyvitamin D3.
- The physicochemical and immunological characteristics of rat lymph binding protein for 25-hydroxyvitamin D3.
- Changes in the concentrations of vitamin D and its metabolites in the plasma of healthy subjects orally given physiological doses of vitamin D2 by multivitamin or vitamin D preparations.
- High-performance liquid chromatographic determination of vitamin D in foods, feeds and pharmaceuticals by successive use of reversed-phase and straight-phase columns.
- High-performance liquid chromatographic determination of vitamin D3 in fish liver oils and eel body oils.
- Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrhosis.