医用生体材料の腹腔滲出マクロファージに及ぼす影響

概要

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Four pulverized bone substitutes for practical use; Alumina (Al), Hydroxyapatite (HAP), Titanium (Ti) and Silica (Si) were studied from the viewpoint of phagocytic activity and cytotoxityin vitroby utilizing rat peritoneal macrophages. In addition to the activation of lysosomal enzyme, the activity of acid phosphatase released from macrophages in the incubation medium were measured after incubating in the presence of those four powderin vitro. We made an attempt to evaluate the biocompatibility of the materials objectively in this study. The results obtained were as follows; 1. In Al and Ti group, the rates of adhesion and viability of macrophages, exhibited the same time courses of changes as the control. In Si group, the rates were the lowest at 120 minutes after incubation. HAP group showed moderate rates between Al, Ti group and Si group. 2. Scanning electron microscopy revealed that some cytoplasmic vacuoles appeared in macrophage of Si and HAP group. Any significant morphological changes were not observed in Al and Ti group. 3. Si group showed the highest value of acid phosphatase activity in four groups. In HAP group the activity was lower than those in Al and Ti groups. Based on the above findings, it was clarified that the effect of such bone substitute on cellular injury increased gradually in order of Al, Ti, HAP and Si. It might be considered that our test can be a promising can didate as a screening test of bone substitutes.