高感度ヘモグロビン測定法による口腔内出血の定量的分析 : 第2報感度の増大とサンプル調製のための新しい舎嗽法の開発

概要

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Since 1966, the cyanmethemoglobin method has been the internationally standardized method for determination of hemoglobin in blood.<BR>However this method is not suitable for determination of low concentrations of hemoglobin in saliva. Recently we devised a modified cyanmethemoglobin method for determination of low concentrations of hemoglobin. In this method, absorption maximum at 420nm having high molecular extinction coefficient was chosen, and sample volume was increased ten-fold.<BR>In the present study, sensitivity was further incresed by a 100-fold increase in sample volume. Thus, hemoglobin concentration could be correctly determined even in 10, 000-fold diluted blood with saline. The use of half-diluted saliva as a diluent showed very little effect on the values of hemoglobin concentration.<BR>For quantitative analysis of intraoral hemorrhage, we adopted a gargling method; after letting a patient gargle with 3 ml of physiological saline for 15 sec, and the saliva mixed with the saline was obtained for hemoglobin assay. Patiens easily understood the method after brief explanations, and approximately the same volumes of the sample as the initial saline were recovered after gargling. The sample could be stored for 12 hours at 5°C or 24 hours at-30°C without significant changes in hemoglobin concentration. The present method seems a good quantitaive analysis for intraoral hemorrhage.