A high sensitive method for determination of hemoglobin applicable to quantitative analysis in oral bleeding.

概要

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Hemostatic condition after oral surgery is now confirmed by subjective methods such as visual observation or listening to patient's complaints. Quantitative analysis of intraoral hemorrhage, however is desirable for the postoperative managment, especially in cases of hemophilia or other coagulopathies. Although determination of hemoglobin concentration in saliva is useful for this purpose, the cyanmethemoglobin method, the internationally standardized method for the determination of hemoglobin in blood, is not suitable for the determination of low concentration of hemoglobin. In this study, a modified cyanmethemoglobin method was devised for the determination of low concentration of hemoglobin. An absorption maximum at 420nm having high molecular extinction coefficient was chosen for the determination of cyanmethemoglobin concentration in the present method, and sample volume was increased ten-fold. Thus, the sensitivity was increased approximately 100-fold in this method, and hemoglobin concentration could be correctly determined even in 1000-fold diluted blood with saline. The use of half-diluted saliva as a diluent showed a little effect on the values of hemoglobin concentration. Although further study is necessary for sampling and/or storage of saliva contaminated with blood, the present method appears to be useful for quantitative analysis of intraoral hemorrhage.
社団法人日本口腔外科学会の論文